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View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.
Emission Color | Green |
---|---|
Brightness | |
λabs | 496 nm |
λem | 516 nm |
Extinction Coefficient (ε) | 76,000 M-1cm-1 |
Quantum Yield (φ) | 0.9 |
Closest Laser line | 488 nm |
Reactive Group | NHS ester |
Reactivity | Primary amines |
pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
Correction Factor 280 | 0.12 |
Application | Imaging, acidic organelle probe, flow, STED |
Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 509.37 |
公式 | C25H13F2NO9 |
储存 | Store at -20°C |
CAS Number | 198139-51-4 |
PubChem ID | 58720805 |
InChI Key | RPOLNGNGJOEMSR-UHFFFAOYSA-N |
Smiles | OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
参考文献是支持产品生物活性的出版物。
Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851
关键词: OG 488, SE, OG 488, SE supplier, OG488SE, green, fluorescent, dye, NHS, ester, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6257, Tocris Bioscience
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Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: