PEI STAR-Go™ transfection reagent

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说明: Aqueous solution of polyethylenimine (PEI) transfection reagent
化学名: Poly[imino(1,2-ethanediyl)] hydrochloride
说明书
引用文献
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实验方案 (6)

生物活性

PEI STAR-Go™ is a ready-to-use aqueous formulation of our high performance PEI STAR™ transfection reagent (Cat. No. 7854), provided as a sterile-filtered solution. PEI STAR™ is a chemically-defined, linear polyethylenimine (PEI) transfection reagent for affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293, CHO, and a range of other eukaryotic cell lines. Applications include production of lentivirus and rAAV, recombinant proteins, and antibodies. Supplied as a 1mg/mL aqueous solution

PEI STAR-Go is a trademark of Bio-Techne Corp.

技术数据

储存 Store at +4°C
CAS Number 49553-93-7

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据

溶解性 Soluble in Aqueous solution (supplied pre-dissolved at a concentration of 1 mg/mL)

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参考文献

参考文献是支持产品生物活性的出版物。

Boussif et al (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc.Natl.Acad.Sci.U.S.A. 92 7297 PMID: 7638184

Longo et al (2013) Transient mammalian cell transfection with polyethylenimine (PEI). Methods Enzymol. 529 227 PMID: 24011049

Huang et al (2013) AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications. J.Virol.Methods 193 270 PMID: 23791963

Trivedi et al (2021) Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Mol.Ther.Methods Clin.Dev. 24 154 PMID: 35071688

Han et al (2021) Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease. Cell Rep. 37 110102 PMID: 34879266


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PEI STAR-Go™ transfection reagent 实验方案

The following protocols feature additional information for the use of PEI STAR-Go™ transfection reagent (Cat. No. 8174).