Polybrene

Cat. No. 7711 1 次引用提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Viral transduction enhancer

别名： Hexadimethrine bromide

化学名： 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide

纯度： ≥95%

引用文献 (1)

实验方案 (1)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Polybrene

Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells in vitro. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols.

Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).

For further information view the product protocol.

技术数据 for Polybrene

储存	Store at +4°C
纯度	≥95%
CAS Number	28728-55-4
Smiles	C[N+](C)(CCC[N+](C)(CCCCCCC)C)C.[Br-].[Br-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Polybrene

溶解性	Soluble to 100 mg/ml in water

产品说明书 for Polybrene

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Polybrene

参考文献是支持产品生物活性的出版物。

Davis et al (2002) Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys.Chem. 97 159 PMID: 12050007

Abe et al (1998) Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 5 708 PMID: 9797877

Shayakhmetov et al (2000) Dependence of adenovirus infectivity on length of the fiber shaft domain. J.Virol. 74 10274 PMID: 11044071

Bolis et al (2021) Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression. Nat.Commun. 12 7033 PMID: 34857732

Zhao et al (2014) Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene. PLoS One 21 e92908 PMID: 24658746

Navarro-Guerrero et al (2021) Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Sci.Rep. 11 4245 PMID: 33608581

Strack (2022) Breaking entry-and species barriers: LentiBOOST ® Plus polybrene enhances transduction efficacy of dendritic cells and monocytes by adenovirus 5. Viruses 14 92 PMID: 35062296

Kumar et al (2021) Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applications. STAR Protoc 2 100874 PMID: 34746857

Joung et al (2022) CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat.Commun 13 1606 PMID: 35338135

Elegheert et al (2018) Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat.Protoc. 13 2991 PMID: 30455477

If you know of a relevant reference for Polybrene, please let us know.

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关键词: Polybrene, Polybrene supplier, transduction, transfection, retrovirus, enhancers, gene, delivery, enhances, viral, Hexadimethrine, bromide, Viral, Transduction, Enhancers, Biochemicals, and, Molecular, Biology, 7711, Tocris Bioscience

1 篇 Polybrene 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Polybrene 的部分引用包括：

Yoshitasu et al (2023) Loss of PI3k activity of inositol polyphosphate multikinase impairs PDK1-mediated AKT activation, cell migration, and intestinal homeostasis. iScience 26 106623 PMID: 37216099

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Polybrene 实验方案

以下实验方案包含使用 Polybrene（目录编号 7711）的更多信息。

Enhanced Transfection of Mammalian Cells with Polybrene

该领域的文献

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

Stem Cell Research Product Guide

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