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preQ1-azide

Cat. No. 7833 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： preQ1 with azide handle for click chemistry
化学名： N-[6-[[(2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]amino]hexyl]-3-(2-azidoethoxy)propanamide
纯度： ≥95% (HPLC)
说明书
引用文献
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for preQ1-azide

preQ1-azide is a modified analog of the natural substrate prequeosine1 (preQ1). It contains an azide handle allowing attachment of additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. preQ1-azide is a toolbox compound for RNA-TAG (transglycosylation at guanosine).

Other RNA-TAG toolbox compounds include preQ1-biotin (Cat. No. 7804) and preQ1- alkyne (Cat. No. 7806)

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

许可信息

Sold under license from The Regents of the University of California.

技术数据 for preQ1-azide

分子量 419.49
公式 C18H29N9O3
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key YYJIXWDJSKVSGF-UHFFFAOYSA-N
Smiles O=C1C2=C(NC=C2CNCCCCCCNC(CCOCCN=[N+]=[N-])=O)N=C(N)N1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for preQ1-azide

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 41.95 100
ethanol 20.97 50

制备储备液 for preQ1-azide

以下数据基于产品分子量 419.49。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 2.38 mL 11.92 mL 23.84 mL
5 mM 0.48 mL 2.38 mL 4.77 mL
10 mM 0.24 mL 1.19 mL 2.38 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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参考文献 for preQ1-azide

参考文献是支持产品生物活性的出版物。

Zhang et al (2022) Site-specific and enzymatic cross-linking of sgRNA enables wavelength-selectable photoactivated control of CRISPR gene editing. J.Am.Chem.Soc. 144 4487 PMID: 35257575

If you know of a relevant reference for preQ1-azide, please let us know.

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关键词: preQ1-azide, preQ1-azide supplier, preQ1, azide, handle, click, chemistry, photoactivatable, probes, RNA, TAG, RNATAG, CuAAC, copper-catalyzed, catalysed, Cu(I), Biochemicals, and, Molecular, Biology, 7833, Tocris Bioscience

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