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preQ1-biotin

Cat. No. 7804 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： PreQ1 with biotin handle for RNA labeling and purification
化学名： N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
纯度： ≥95% (HPLC)
说明书
引用文献
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生物活性 Scientific Data 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for preQ1-biotin

preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

Scientific Data

Structure Schematic representation of preQ1-bitoin insertion into DNA. View Larger

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin. Schematic representation of preQ1-bitoin insertion into DNA.

Data Urea-PAGE gel shift analysis of preQ1-biotin insertion into a DNA View Larger

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin. Urea-PAGE gel shift analysis of preQ1-biotin insertion. Modification is evidenced by an upward shift of the product band due to molecular weight increase. Lane 1 is unmodified DNA; lane 2 is preQ1-biotin modified DNA.

许可信息

Sold under license from The Regents of the University of California.

技术数据 for preQ1-biotin

分子量 504.65
公式 C23H36N8O3S
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 2971850-25-4
InChI Key APUALFAJXKNNBF-BXWFABGCSA-N
Smiles O=C1C2=C(NC=C2CNCCCCCCNC(CCCC[C@H]3[C@]4([H])[C@](CS3)(NC(N4)=O)[H])=O)N=C(N)N1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for preQ1-biotin

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 10.09 20
ethanol 2.52 5

制备储备液 for preQ1-biotin

以下数据基于产品分子量 504.65。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.2 mM 9.91 mL 49.54 mL 99.08 mL
1 mM 1.98 mL 9.91 mL 19.82 mL
2 mM 0.99 mL 4.95 mL 9.91 mL
10 mM 0.2 mL 0.99 mL 1.98 mL

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参考文献 for preQ1-biotin

参考文献是支持产品生物活性的出版物。

Busby et al (2020) Enzymatic RNA biotinylation for affinity purification and identification of RNA-protein interactions. ACS Chem.Biol. 15 2247 PMID: 32706237

Tota and Devaraj (2023) Site-specific covalent labeling of DNA substrates by an RNA transglycosylase. J.Am.Chem.Soc 145 8099 PMID: 36988146

If you know of a relevant reference for preQ1-biotin, please let us know.

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关键词: preQ1-biotin, preQ1-biotin supplier, preq1biotin, prequeosine1, RNA, binding, labeling, purification, TGT, rna, tag, rnatag, rna-tag, dna-tag, dnatag, Biochemicals, and, Molecular, Biology, 7804, Tocris Bioscience

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