ProtiFect STAR™ Protein Transfection Reagent

Cat. No. 8098 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Protein transfection reagent

实验方案 (1)

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性

ProtiFect STAR™ Protein Transfection Reagent is a transfection reagent capable of delivering a wide range of proteins with varying molecular weights (up to 240 kDa) and isoelectric points to the cytoplasm, including Cas9 ribonucleoproteins and BSA-FITC. ProtiFect STAR™ is a gemini amphiphile, which bypasses endocytic pathways. Can be used in vivo and in vitro in a variety of cell types including primary cells, T-cells, B-cells and stem cells. For more information on how to use ProtiFect STAR™, please see our protocol.

This compound is supplied pre-dissolved in ethanol (10 mg/mL).

Scientific Data

ProtiFect STAR™ Protein transfection reagent applications

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Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of negatively charged green fluorescence protein (GFP), superoxide dismutase (SOD), ovalbumin (OVA), and positively charged lysozyme with a commercially available protein transfection reagent and ProtiFect STAR™. Proteins and ProtiFect STAR™ were at 8 μg/mL (n = 3). Scale = 25 μm.

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Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of CRISPR/Cas9 ribonucleoprotein complexes with ProtiFect STAR™ in 293T cells targeting adeno-associated virus integration site 1 (AAVS1), leading to an indel frequency of 37.8%.

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Application of ProtiFect STAR™ Protein Transfection Reagent. Intracellular delivery of CRISPR/Cas9 ribonucleoprotein complexes with ProtiFect STAR™ in SW-480 cells targeting KRAS locus. CMAX was used as a positive control. Sanger sequence (lower panel) performed by T-A cloning at KRAS locus was obtained from SW-480 cells. Three sequences of clones with mutations were represented. Cas9 protein, sgRNA, and ProtiFect STAR™ were 2, 1, and 4 μg/mL, respectively.

技术数据

储存	Store at -20°C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据

溶解性	Soluble in ethanol (supplied pre-dissolved in anhydrous ethanol, 10mg/ml)

产品说明书

分析证书/产品说明书

化学品安全技术说明书

参考文献

参考文献是支持产品生物活性的出版物。

Le et al (2023) Direct cytosolic delivery of proteins and CRISPR-Cas9 genome editing by gemini amphiphiles via non-endocytic translocation pathways. ACS Cent.Sci. 9 1313 PMID: 37521791

Zhao et al (2023) Gemini amphiphiles: robust cytosolic delivery of therapeutic biomacromolecules through non-endocytic translocation pathways. AC Cent.Sci. 9 1266 PMID: 37521797

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