TFAX 488, TFP

Discontinued Product

6621 has been discontinued.

View all TFAX (AF) Dyes products.
说明: Green fluorescent dye; amine reactive
化学名: 3,6-Diamino-9-[2-carboxy-4-[(2,3,5,6-tetrafluorophenoxy)carbonyl]phenyl]-4,5-disulfoxanthylium bis(triethylammmonium) salt
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生物活性 for TFAX 488, TFP

TFAX 488, TFP is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.

Optical Data for TFAX 488, TFP

TFAX 488, TFP Dye Spectra
Emission Color Green
Brightness
λabs 495 nm
λem 515 nm
Extinction Coefficient (ε) 73,000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group TFP
Reactivity Primary amines
Cell Permeable No
pH Sensitivity pH insensitive pH 4-10
Application flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED
Alternative For Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

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技术数据 for TFAX 488, TFP

分子量 884.91
公式 C27H14F4N2O11S2.C12H30N2
储存 Store at -20°C
CAS Number 878546-79-3
Smiles CCN(CC)CC.CCN(CC)CC.[NH2+]=C1C=CC2=C(C3=CC=C(C(OC4=C(F)C(F)=CC(F)=C4F)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)(O)=O)=C5OC2=C1S(=O)(O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

参考文献 for TFAX 488, TFP

参考文献是支持产品生物活性的出版物。

Schilling et al (2006) On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry 45 12393 PMID: 17029395

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Fluorescent Dyes and Probes Research Product Guide

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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

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