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说明： Cell-permeable Zn2+ chelator; also inhibits RNA binding protein Lin28
化学名： N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine
纯度： ≥97% (HPLC)
生物活性 for TPEN
TPEN is a heavy metal chelator. Reacts with both Zn-proteome and Zn-metallothionein (MT) in LLC-PK1 cells; acts as an intracellular chelator of proteomic Zn2+. Activity decreases intracellular zinc levels, and induces apoptosis in HeLa and cultured human retinal pigment epithelium (RPE) cells. Also inhibits RNA binding protein Lin28 (IC50 = 2.5 μM). Cell permeable.
技术数据 for TPEN
| 分子量 | 424.54 |
| 公式 | C26H28N6 |
| 储存 | Store at +4°C |
| 纯度 | ≥97% (HPLC) |
| CAS Number | 16858-02-9 |
| PubChem ID | 5519 |
| InChI Key | CVRXLMUYFMERMJ-UHFFFAOYSA-N |
| Smiles | N(CC3=CC=CC=N3)(CC4=CC=CC=N4)CCN(CC2=CC=CC=N2)CC1=CC=CC=N1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for TPEN
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 10.61 | 25 | |
| ethanol | 42.45 | 100 |
制备储备液 for TPEN
以下数据基于产品分子量 424.54。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.36 mL | 11.78 mL | 23.55 mL |
| 5 mM | 0.47 mL | 2.36 mL | 4.71 mL |
| 10 mM | 0.24 mL | 1.18 mL | 2.36 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.47 mL |
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产品说明书 for TPEN
参考文献 for TPEN
参考文献是支持产品生物活性的出版物。
Chimienti et al (2001) Role of cellular zinc in programmed cell death: temporal relationship between zinc depletion, activation of caspases, and cleavage of Sp family transcription factors. Biochem.Pharmacol. 62 51 PMID: 11377396
Rana et al (2008) Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. J.Inorg.Biochem. 102 489 PMID: 18171589
Matias et al (2010) Validation of TPEN as a zinc chelator in fluorescence probing of calcium in cells with the indicator Fura-2. J.Fluoresc. 20 377 PMID: 19821015
Hyun et al (2001) Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells. Invest.Opthalmol.Vis.Sci. 42 460
Wang et al (2018) Small-molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28. Cell Rep. 23 3091 PMID: 29874593
If you know of a relevant reference for TPEN, please let us know.
关键词: TPEN, TPEN supplier, zinc, zn2+, chelating, chelators, cell, permeable, permeant, heavy, metals, Biochemicals, and, Molecular, Biology, DNA,, RNA, Protein, Synthesis, 4309, Tocris Bioscience
3 篇 TPEN 的引用文献
引用文献是使用了 Tocris 产品的出版物。 TPEN 的部分引用包括：
Kerns (2018) Zinc ion flux during mammalian sperm capacitation. Nat Commun 9 2061 PMID: 29802294
Tong et al (2022) The Role of Transient Receptor Potential A1 and G Protein-Coupled Receptor 39 in Zinc-Mediated Acute and Chronic Itch in Mice. Front Mol Neurosci 14 768731 PMID: 35095413
Mustafa et al (2023) Curcumin attenuates hydroxychloroquine-mediated apoptosis and oxidative stress via the inhibition of TRPM2 channel signalling pathways in a retinal pigment epithelium cell line. Graefes Arch Clin Exp Ophthalmol PMID: 37099129
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