Home / Pharmacology / Enzymes / Kinases / MEK / MEK Inhibitors / U0126

U0126

Cat. No. 1144 131 Citations 4 Reviews Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Potent, selective inhibitor of MEK1 and 2
Chemical Name: 1,4-Diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene
Datasheet
Citations (131)
Reviews (4)
Literature (1)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for U0126

U0126 is a potent and selective non-competitive inhibitor of MAP kinase kinase. Inhibits MEK-1 and MEK-2 (IC50 values of 0.07 and 0.06 μM respectively) with little or no effect on the activities of PKC, Abl, Raf, MEKK, ERK, JNK, MKK-3, MKK-4/SEK, MKK-6, Cdk2 or Cdk4. Brain penetrant. Helps maintain human pluripotent stem cells in undifferentiated state.

Negative Control also available.

Technical Data for U0126

M. Wt 380.48
Formula C18H16N6S2
Storage Desiccate at +4°C
CAS Number 109511-58-2
PubChem ID 3006531
InChI Key DVEXZJFMOKTQEZ-JYFOCSDGSA-N
Smiles N\C(SC1=CC=CC=C1N)=C(C#N)/C(C#N)=C(\N)SC1=CC=CC=C1N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for U0126

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.05 100

Preparing Stock Solutions for U0126

The following data is based on the product molecular weight 380.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.63 mL 13.14 mL 26.28 mL
5 mM 0.53 mL 2.63 mL 5.26 mL
10 mM 0.26 mL 1.31 mL 2.63 mL
50 mM 0.05 mL 0.26 mL 0.53 mL

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Product Datasheets for U0126

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for U0126

References are publications that support the biological activity of the product.

Davies et al (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 351 95 PMID: 10998351

Duncia et al (1998) MEK inhibitors: the chemistry and biological activity of U0126, its analogs, and cyclization products. Bioorg.Med.Chem.Lett. 8 2839 PMID: 9873633

Favata et al (1998) Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J.Biol.Chem. 273 18623 PMID: 9660836

Namura et al (2001) Intravenous administraton of MEK inhibitor U0126 affords brain protection against forebrain ischemia and focal cerebral ischemia. Proc.Natl.Acad.Sci.U.S.A. 98 11569 PMID: 11504919

Kinehara et al (2013) Protein kinase C regulates human pluripotent stem cell self-renewal. PLoS One 8 e54122 PMID: 23349801

If you know of a relevant reference for U0126, please let us know.

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Keywords: U0126, U0126 supplier, Potent, selective, inhibitors, inhibits, MEK1, MEK2, Mitogen-Activated, Protein, Kinase, Kinases, MEK, Stem, Cell, Proliferation, 1144, Tocris Bioscience

131 Citations for U0126

Citations are publications that use Tocris products. Selected citations for U0126 include:

Guo et al (2016) Selective Disruption of Metabotropic Glutamate Receptor 5-Homer Interactions Mimics Phenotypes of Fragile X Syndrome in Mice. J Neurosci 36 2131 PMID: 26888925

Tuvikene et al (2016) AP-1 Transcription Factors Mediate BDNF-Positive Feedback Loop in Cortical Neurons. J Neurosci 36 1290 PMID: 26818516

Sovadinova et al (2015) Phosphatidylcholine Specific PLC-Induced Dysregulation of Gap Junctions, a Robust Cellular Response to Environmental Toxicants, and Prevention by Resveratrol in a Rat Liver Cell Model. Oncogene 10 e0124454 PMID: 26023933

Zhao et al (2015) IL-13Rα2 mediates PNR-induced migration and metastasis in ERα-negative breast cancer. Nature 34 1596 PMID: 24747967

Do-Monte et al (2015) A temporal shift in the circuits mediating retrieval of fear memory. J Neuroinflammation 519 460 PMID: 25600268

Liu et al (2015) Hyperphosphate-Induced Myocardial Hypertrophy through the GATA-4/NFAT-3 Signaling Pathway Is Attenuated by ERK Inhibitor Treatment. Mol Cancer 5 79 PMID: 25999956

Koutsioumpa et al (2015) Receptor protein tyrosine phosphatase β/ζ is a functional binding partner for vascular endothelial growth factor. J Neurosci 14 19 PMID: 25644401

Briz et al (2015) Activity-dependent rapid local RhoA synthesis is required for hippocampal synaptic plasticity. Exp Hematol Oncol 35 2269 PMID: 25653381

Koerber et al (2015) Analysis of the anti-proliferative and the pro-apoptotic efficacy of Syk inhibition in multiple myeloma. Int J Cancer 4 21 PMID: 26251761

Choi et al (2015) SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II. J Neurosci 136 E219 PMID: 25137150

Morse et al (2013) Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family. BMC Pharmacol Toxicol 14 17 PMID: 23497702

Nemoto et al (2013) Angiotensin II produces nociceptive behavior through spinal AT1 receptor-mediated p38 mitogen-activated protein kinase activation in mice. Mol Pain 9 38 PMID: 23898828

Huang et al (2010) Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor. J Neurosci 30 6080 PMID: 20427666

Huang and Hsu (2010) Activation of muscarinic acetylcholine receptors induces a nitric oxide-dependent long-term depression in rat medial prefrontal cortex. Cereb Cortex 20 982 PMID: 19666830

Chen et al (2010) Ca(v)3.2 T-type Ca2+ channel-dependent activation of ERK in paraventricular thalamus modulates acid-induced chronic muscle pain. J Neurosci 30 10360 PMID: 20685979

Bellik et al (2005) Intracellular pathways triggered by the selective FLT-1-agonist placental growth factor in vascular smooth muscle cells exposed to hypoxia. Br J Pharmacol 146 568 PMID: 16086034

Devocelle et al (2019) Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis. Int J Cell Biol 2019 9151394 PMID: 31360169

Christianson et al (2014) Learned stressor resistance requires extracellular signal-regulated kinase in the prefrontal cortex. Front Behav Neurosci 8 348 PMID: 25324750

Breshears et al (2012) A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem 287 32578 PMID: 22833676

Gangoiti et al (2012) Ceramide 1-phosphate stimulates proliferation of C2C12 myoblasts. Biochimie 94 597 PMID: 21945811

Schulz et al (2012) Phenotype-based high-content chemical library screening identifies STAT as inhibitors of in vivo lymphangiogenesis. Proc Natl Acad Sci U S A 109 E2665 PMID: 22949700

Arora et al (2012) Expression of protein-tyrosine phosphatases in Acute Myeloid Leukemia cells: FLT3 ITD sustains high levels of DUSP6 expression. Cell Commun Signal 10 19 PMID: 22784513

Hodges et al (2012) Signaling pathways used by EGF to stimulate conjunctival goblet cell secretion. Exp Eye Res 103 99 PMID: 22975404

Lee et al (2012) Fucoidan from seaweed Fucus vesiculosus inhibits migration and invasion of human lung cancer cell via PI3K-Akt-mTOR pathways. PLoS One 7 e50624 PMID: 23226337

Le et al (2010) The glutamate release inhibitor R.zole decreases migration, invasion, and proliferation of melanoma cells. J Invest Dermatol 130 2240 PMID: 20505744

Melemedjian et al (2010) IL-6- and NGF-induced rapid control of protein synthesis and nociceptive plasticity via convergent signaling to the eIF4F complex. J Neurosci 30 15113 PMID: 21068317

Beshir et al (2010) Raf kinase inhibitor protein suppresses nuclear factor-κB-dependent cancer cell invasion through negative regulation of matrix metalloproteinase expression. Cancer Lett 299 137 PMID: 20855151

Ishida et al (2010) Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish. Dev Biol 340 468 PMID: 20144602

He and Aizenman (2010) ERK signaling leads to mitochondrial dysfunction in extracellular zinc-induced neurotoxicity. J Neurochem 114 452 PMID: 20412391

Braun et al (2010) Uridine triphosphate (UTP) induces profibrotic responses in cardiac fibroblasts by activation of P2Y2 receptors. J Mol Cell Cardiol 49 362 PMID: 20471392

Chotiner et al (2010) Assessment of the role of MAP kinase in mediating activity-dependent transcriptional activation of the immediate early gene Arc/Arg3.1 in the dentate gyrus in vivo. Learn Mem 17 117 PMID: 20154358

Ito et al (2010) Synergistic growth inhibition of anaplastic large cell lymphoma cells by combining cellular ALK gene silencing and a low dose of the kinase inhibitor U0126. Cancer Gene Ther 17 633 PMID: 20448669

Alao et al (2006) Role of glycogen synthase kinase 3 β (GSK3β) in mediating the cytotoxic effects of the histone deacetylase inhibitor trichostatin A (TSA) in MCF-7 breast cancer cells. J Neurosci 5 40 PMID: 17018141

O'Riordan et al (2006) Regulation of nuclear factor κB in the hippocampus by group I metabotropic glutamate receptors. Cell Host Microbe 26 4870 PMID: 16672661

Kauppinen et al (2006) Direct phosphorylation and regulation of poly(ADP-ribose) polymerase-1 by extracellular signal-regulated kinases 1/2. Proc Natl Acad Sci U S A 103 7136 PMID: 16627622

Pietak et al (2019) Neural control of body-plan axis in regenerating planaria. PLoS Comput Biol 15 e1006904 PMID: 30990801

Szybowska et al (2019) Cancer Mutations in FGFR2 Prevent a Negative Feedback Loop Mediated by the ERK1/2 Pathway. Cells 8 518 PMID: 31146385

Yumnam et al (2014) hesperidin induces paraptosis like cell death in hepatoblastoma, HepG2 Cells: involvement of ERK1/2 MAPK [corrected]. PLoS One 9 e101321 PMID: 24977707

Grell et al (2014) Regulatory mechanism of endothelin receptor B in the cerebral arteries after focal cerebral ischemia. PLoS One 9 e113624 PMID: 25479176

Emery et al (2014) Separate cyclic AMP sensors for neuritogenesis, growth arrest, and survival of neuroendocrine cells. J Biol Chem 289 10126 PMID: 24567337

Heckler et al (2014) ERK/MAPK regulates ERRγ expression, transcriptional activity and receptor-mediated tamox. resistance in ER+ breast cancer. FEBS J 281 2431 PMID: 24684682

Grzmil et al (2014) MNK1 pathway activity maintains protein synthesis in rapalog-treated gliomas. J Clin Invest 124 742 PMID: 24401275

Guemez-Gamboa et al (2014) Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching. Neuron 82 1004 PMID: 24908484

Pera et al (2010) Cigarette smoke and lipopolysaccharide induce a proliferative airway smooth muscle phenotype. J Neurosci 11 48 PMID: 20429916

Kolber et al (2010) Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. Nat Commun 30 8203 PMID: 20554871

Liu et al (2007) Angiotensin II inhibits native bTREK-1 K+ channels through a PLC-, kinase C-, and PIP2-independent pathway requiring ATP hydrolysis. Am J Physiol Cell Physiol 293 C682 PMID: 17494631

Huang et al (2007) Actin polymerization and ERK phosphorylation are required for Arc/Arg3.1 mRNA targeting to activated synaptic sites on dendrites. J Neurosci 27 9054 PMID: 17715342

Schaafsma et al (2007) Ins induces airway smooth muscle contraction. J Biomed Sci 150 136 PMID: 17160007

Khan et al (2007) Catecholaminergic control of mitogen-activated protein kinase signaling in paraventricular neuroendocrine neurons in vivo and in vitro: a proposed role during glycemic challenges. Nat Commun 27 7344 PMID: 17611287

Domin et al (2019) Characterization of the brain penetrant neuropeptide Y Y2 receptor antagonist SF-11. ACS Chem Neurosi 10 3454 PMID: 31267743

Zhang et al (2016) Bromodomain containing protein represses the Ras/Raf/MEK/ERK pathway to attenuate human hepatoma cell proliferation during HCV infection. Cancer Lett. 371 107 PMID: 26620707

Yang et al (2016) The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages. Nat Commun 7 11385 PMID: 27150562

Lee et al (2016) Mincle-mediated translational regulation is required for strong nitric oxide production and inflammation resolution. Cardiorenal Med 7 11322 PMID: 27089465

Dang et al (2016) Apocynin prevents mitochondrial burdens, microglial activation, and pro-apoptosis induced by a toxic dose of metha. in the striatum of mice via inhibition of p47phox activation by ERK. Korean J Physiol Pharmacol 13 12 PMID: 26780950

Dohgu et al (2011) Lipopolysaccharide-enhanced transcellular transport of HIV-1 across the blood-brain barrier is mediated by luminal microvessel IL-6 and GM-CSF. Br J Pharmacol 8 167 PMID: 22129063

Hsu et al (2011) JNK suppression is essential for 17β-OE inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells. Cell Commun Signal 18 61 PMID: 21859479

Mou et al (2011) Rapid brain-derived neurotrophic factor-dependent sequestration of amygdala and hippocampal GABA(A) receptors via different tyrosine receptor kinase B-mediated phosphorylation pathways. Respir Res 176 72 PMID: 21195749

Kim et al (2011) Thrombin-induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells. Mol Cancer 15 211 PMID: 21994479

Kovac et al (2011) Brain microvascular pericytes are immunoactive in culture: cytokine, chemokine, nitric oxide, and LRP-1 expression in response to lipopolysaccharide. J Neurosci 8 139 PMID: 21995440

Lim et al (2008) The novel adipocytokine visfatin exerts direct cardioprotective effects. J Cell Mol Med 12 1395 PMID: 18400051

Coba et al (2008) Kinase networks integrate profiles of N-MthD.-aspartate receptor-mediated gene expression in hippocampus. J Biol Chem 283 34101 PMID: 18815127

Lourenco et al (2015) Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors. J Immunol 194 3463 PMID: 25712213

Tang et al (2015) P2X7 Receptor Regulates Internalization of Antimicrobial Peptide LL-37 by Human Macrophages That Promotes Intracellular Pathogen Clearance. J Immunol 195 1191 PMID: 26116509

Schubert et al (2014) Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis. Acta Neuropathol 128 835 PMID: 25341622

Babu et al (2014) TGFβ-mediated suppression of CD248 in non-cancer cells via canonical Smad-dependent signaling pathways is uncoupled in cancer cells. BMC Cancer 14 113 PMID: 24555435

Wan et al (2014) Cathelicidin LL-37 induces time-resolved release of LTB4 and TXA2 by human macrophages and triggers eicosanoid generation in vivo. FASEB J 28 3456 PMID: 24736410

Meyer et al (2013) Distinct dedifferentiation processes affect caveolin-1 expression in hepatocytes. J Pharmacol Exp Ther 11 6 PMID: 23339737

Philips et al (2013) MAPK establishes a molecular context that defines effective training patterns for long-term memory formation. J Neurosci 33 7565 PMID: 23616561

Jensen et al (2013) An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis during coxsackievirus B3 infection. J Neurosci 13 67 PMID: 23332156

Ito et al (2011) Interleukin-2 Functions in Anaplastic Large Cell Lymphoma Cells through Augmentation of Extracellular Signal-Regulated Kinases 1/2 Activation. Int J Biomed Sci 7 181 PMID: 23675235

Sbroggiò et al (2011) ERK1/2 activation in heart is controlled by melusin, focal adhesion kinase and the scaffold protein IQGAP1. J Cell Sci 124 3515 PMID: 22010199

Meitzen et al (2011) β1-Adrenergic receptors activate two distinct signaling pathways in striatal neurons. J Neurochem 116 984 PMID: 21143600

Melemedjian et al (2011) Targeting adenosine monophosphate-activated protein kinase (AMPK) in preclinical models reveals a potential mechanism for the treatment of neuropathic pain. Mol Pain 7 70 PMID: 21936900

Rubert et al (2011) Bim and Mcl-1 exert key roles in regulating JAK2V617F cell survival. BMC Cancer 11 24 PMID: 21247487

Dohgu and Banks (2008) Lipopolysaccharide-enhanced transcellular transport of HIV-1 across the blood-brain barrier is mediated by the p38 mitogen-activated protein kinase pathway. Exp Neurol 210 740 PMID: 18295207

Collar et al (2018) The homozygous CX3CR1-M280 mutation impairs human monocyte survival. JCI Insight 3 e95417 PMID: 29415879

Andersson et al (2018) Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis. JCI Insight 3 e122375 PMID: 30333314

Zhang et al (2018) Integrin CD11b negatively regulates Mincle-induced signaling via the Lyn-SIRPα-SHP1 complex. Exp Mol Med 50 e439 PMID: 29400702

Lin et al (2018) Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells. Cancer Manag Res 10 4237 PMID: 30323679

Liang et al (2014) δ-Opioid receptors up-regulate excitatory amino acid transporters in mouse astrocytes. J Neuroinflammation 171 5417 PMID: 25052197

Alpár et al (2014) Endocannabinoids modulate cortical development by configuring Slit2/Robo1 signalling. Cell Rep 5 4421 PMID: 25030704

Wan et al (2014) Retinal injury, growth factors, and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration. Neuroscience 9 285 PMID: 25263555

Voon et al (2013) EMT-induced stemness and tumorigenicity are fueled by the EGFR/Ras pathway. PLoS One 8 e70427 PMID: 23950932

Li et al (2013) Effect of VIP on intracellular [Ca2+], extracellular regulated kinase 1/2, and secretion in cultured rat conjunctival goblet cells. Invest Ophthalmol Vis Sci 54 2872 PMID: 23518767

Ahmadiantehrani and Ron (2013) DA D2 receptor activation leads to an up-regulation of glial cell line-derived neurotrophic factor via Gβγ-Erk1/2-dependent induction of Zif268. J Neurochem 125 193 PMID: 23373701

Ripple et al (2013) Acute mitochondrial inhibition by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) 1/2 inhibitors regulates proliferation. J Biol Chem 288 2933 PMID: 23235157

Osgood et al (2013) Polycyclic aromatic hydrocarbon-induced signaling events relevant to inflammation and tumorigenesis in lung cells are dependent on molecular structure. PLoS One 8 e65150 PMID: 23755184

Korb et al (2013) Arc in the nucleus regulates PML-dependent GluA1 transcription and homeostatic plasticity. Nat Neurosci 16 874 PMID: 23749147

Lucchetti et al (2013) The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer. PLoS One 8 e55355 PMID: 23390529

Melemedjian et al (2013) mTORC1 inhibition induces pain via IRS-1-dependent feedback activation of ERK. Pain 154 1080 PMID: 23607966

Roselli et al (2011) CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton. PLoS One 6 e23097 PMID: 21829588

Zhao et al (2011) Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus. J Neurosci 31 725 PMID: 21228181

Morse et al (2011) Ligand-directed functional selectivity at the mu opioid receptor revealed by label-free integrative pharmacology on-target. PLoS One 6 e25643 PMID: 22003401

Riedl et al (2009) Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing. PLoS One 29 13377 PMID: 19846725

Saliba et al (2015) Evidence of a Role for Fibroblast Transient Receptor Potential Canonical 3 Ca2+ Channel in Renal Fibrosis. J Am Soc Nephrol 26 1855 PMID: 25479966

Meyer et al (2015) Type 1 Ins-Like Growth Factor Receptor/Ins Receptor Substrate 1 Signaling Confers Pathogenic Activity on Breast Tumor Cells Lacking REST. Mol Cell Biol 35 2991 PMID: 26100015

Li et al (2015) MAPK signaling downstream to TLR4 contributes to PacT.-induced peripheral neuropathy. Brain Behav.Immun. 49 255 PMID: 26065826

Zhang et al (2012) Activation of the Ras/Raf/MEK pathway facilitates hepatitis C virus replication via attenuation of the IF.-JAK-STAT pathway. J Virol 86 1544 PMID: 22114332

Chen et al (2012) Curcumin inhibits HCV replication by induction of heme oxygenase-1 and suppression of AKT. Int J Mol Med 30 1021 PMID: 22922731

Makhortova et al (2011) A screen for regulators of survival of motor neuron protein levels. Nat Chem Biol 7 544 PMID: 21685895

Tsuda et al (2009) Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways. J Cell Mol Med 13 3251 PMID: 19298529

Roselli et al (2009) Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways. PLoS One 4 e6011 PMID: 19547699

Roscioni et al (2009) PKA and Epac cooperate to augment bradykinin-induced interleukin-8 release from human airway smooth muscle cells. Respir Res 10 88 PMID: 19788733

Zhong et al (2009) BC1 regulation of metabotropic glutamate receptor-mediated neuronal excitability. J Neurosci 29 9977 PMID: 19675232

Pintchovski et al (2009) The serum response factor and a putative novel transcription factor regulate expression of the immediate-early gene Arc/Arg3.1 in neurons. J Neurosci 29 1525 PMID: 19193899

Magliaro and Saldanha (2009) Clozapine protects PC-12 cells from death due to oxidative stress induced by hydrogen peroxide via a cell-type specific mechanism involving inhibition of extracellular signal-regulated kinase phosphorylation. Brain Res 1283 14 PMID: 19501068

Roberts (2001) Role of the extracellular signal-regulated kinase (Erk) signal transduction cascade in alpha(2) adrenoceptor-mediated vasoconstriction in porcine palmar lateral vein. Br J Pharmacol 133 859 PMID: 11454659

Ernst et al (2019) Humanized anti-CD123 antibody facilitates NK cell antibody-dependent cell-mediated cytotoxicity (ADCC) of Hodgkin lymphoma targets via ARF6/PLD-1. Blood Cancer J 9 6 PMID: 30647406

Wang et al (2016) Corticotrigeminal Projections from the Insular Cortex to the Trigeminal Caudal Subnucleus Regulate Orofacial Pain after Nerve Injury via Extracellular Signal-Regulated Kinase Activation in Insular Cortex Neurons. Front Cell Neurosci 9 493 PMID: 26733817

Berdichevsky et al (2013) PI3K-Akt signaling activates mTOR-mediated epileptogenesis in organotypic hippocampal culture model of post-traumatic epilepsy. J Neurosci 33 9056 PMID: 23699517

Yan et al (2012) Sensitization of dural afferents underlies migraine-related behavior following meningeal application of interleukin-6 (IL-6). Mol Pain 8 6 PMID: 22273495

Li et al (2012) Hepatitis C virus activates Bcl-2 and MMP-2 expression through multiple cellular signaling pathways. J Virol 86 12531 PMID: 22951829

Ye et al (2012) Local synaptic integration of mitogen-activated protein kinase and protein kinase A signaling mediates intermediate-term synaptic facilitation in Aplysia. Proc Natl Acad Sci U S A 109 18162 PMID: 23071303

Upham et al (2009) Structure-activity-dependent regulation of cell communication by perfluorinated fatty acids using in vivo and in vitro model systems. Environ Health Perspect 117 545 PMID: 19440492

Doulatov et al (2009) PLZF is a regulator of homeostatic and cytokine-induced myeloid development. Genes Dev 23 2076 PMID: 19723763

Park et al (2009) 4-hydroxyOE induces anchorage-independent growth of human mammary epithelial cells via activation of IkappaB kinase: potential role of reactive oxygen species. Cancer Res 69 2416 PMID: 19276383

Gallagher et al (2004) Extracellular signal-regulated protein kinase activation is required for metabotropic glutamate receptor-dependent long-term depression in hippocampal area CA1. J Neuroinflammation 24 4859 PMID: 15152046

Dominguez et al (2004) Morphological effects of estrogen on cholinergic neurons in vitro involves activation of extracellular signal-regulated kinases. J Neurosci 24 982 PMID: 14749443

Pierre et al (2004) PAM mediates sustained inhibition of cAMP signaling by sphingosine-1-phosphate. EMBO J 23 3031 PMID: 15257286

Wang et al (2004) Block of long-term potentiation by naturally secreted and synthetic amyloid beta-peptide in hippocampal slices is mediated via activation of the kinases c-Jun N-terminal kinase, cyclin-dependent kinase 5, and p38 mitogen-activated protein kinase as well a J Neurosci 24 3370 PMID: 15056716

Atherton et al (2003) IL-13-induced changes in the goblet cell density of human bronchial epithelial cell cultures: MAP kinase and phosphatidylinositol 3-kinase regulation. Am J Physiol Lung Cell Mol Physiol 285 L730 PMID: 12794003

Li et al (2021) Oxytocin in the anterior cingulate cortex attenuates neuropathic pain and emotional anxiety by inhibiting presynaptic long-term potentiation Cell Rep 36 109411 PMID: 34289348

Zhen et al (2019) Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5. Biomol Ther (Seoul) 27 395 PMID: 30419635

Suo et al (2019) Inhibition of MAPK/ERK pathway promotes oligodendrocytes generation and recovery of demyelinating diseases. Glia 67 1320 PMID: 30815939

Cagnetta et al (2019) Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73 474 PMID: 30595434

Gillman et al (2017) Epidermal Growth Factor Receptor Signaling Enhances the Proinflammatory Effects of Staphylococcus aureus Gamma-Toxin on the Mucosa. Toxins (Basel) 9 202 PMID: 28657583

Wan and Goldman (2017) Opposing Actions of Fgf8a on Notch Signaling Distinguish Two Muller Glial Cell Populations that Contribute to Retina Growth and Regeneration. Cell Rep 19 849 PMID: 28445734

Wu (2017) TRIB3 downregulation enhances doxorubicin-induced cytotoxicity in gastric cancer cells. Arch Biochem Biophys 622 26 PMID: 28442401

Noskovičová et al (2018) Cub domain-containing protein 1 negatively regulates TGF-β signaling and myofibroblast differentiation. Am J Physiol Lung Cell Mol Physiol 314 L695 PMID: 29351434

Kim et al (2018) Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells. Cell Rep 25 2148 PMID: 30463012

Li et al (2018) Upregulation of IRS1 Enhances IGF1 Response in Y537S and D538G ESR1 Mutant Breast Cancer Cells. Endocrinology 159 285 PMID: 29029116

Do you know of a great paper that uses U0126 from Tocris? Please let us know.

Reviews for U0126

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Experiment.
By Chi Ngo on 09/28/2024
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Gastic epithelical cells (AGS)

To investigate if the induction of Snail1 by Helicobacter pylori infection in gastric epithelial cells AGS was regulated by the MEK/ERK pathway.

Aliquot the reconstituted compound to avoid freezing and thawing repeatedly.

PMID: 26808296 Reference
review image
Used to block MEK signaling..
By MARINA KOUTSIOUMPA on 10/13/2022
Assay Type: In Vitro
Species: Human

Pancreatic cancer cells were pretreated for 2 hours with U0126 followed by conventional chemotherapeutics. MEK inhibition had an additive effect on cancer cell’s viability upon 3 days of treatment.

Used for Migration assays.
By Anonymous on 07/01/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: A549 or MDAMB231

All MAPK inhibitors (ZM336372: c-RAF inhibitor; UO126: MEK1/MEK2 inhibitor, SB203580: p38 inhibitor) were used at 10 μM

We ordered this product, combining with another inhibitor, to confirm the migration.To confirm that tested product induces migration, we also screen all MAPK inhibitors and MIF receptor antagonists

PMID: 25712213
review image
Effect of MEK inhibitor on E0771 extracellular flux.
By Anonymous on 10/10/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: E0771

Extracellular flux analysis (Seahorse experiment) to examine the effect of MEK inhibitor in breast cancer cells. 5-10uM dose used.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

MAPK Signaling Scientific Review

MAPK Signaling Scientific Review

MAP kinase signaling is integral to the regulation of numerous cellular processes such as proliferation and differentiation, and as a result is an important focus of cancer and immunology research. Updated for 2016, this review discusses the regulation of the MAPK pathway and properties of MAPK cascades. Compounds available from Tocris are listed.

Pathways for U0126

MAPK Signaling Pathway

MAPK Signaling Pathway

The mitogen-activated protein kinase pathway evokes an intracellular signaling cascade in response to extracellular stimuli such as heat and stress. It can influence cell division, metabolism and survival.