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Description: AMPK activator
Chemical Name: N1-(β-D-Ribofuranosyl)-5-aminoimidazole-4-carboxamide
Purity: ≥99% (HPLC)
Biological Activity for AICAR
AICAR is a cell-permeable, allosteric activator of AMP-activated protein kinase (AMPK). Augments proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells and attenuates psychosine-induced expression of proinflammatory cytokines and iNOS in astrocytes. Promotes osteogenic differentiation of hAMSCs and BM-MSCs in vitro.
Compound Libraries for AICAR
AICAR is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for AICAR
| M. Wt | 258.23 |
| Formula | C9H14N4O5 |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 2627-69-2 |
| PubChem ID | 17513 |
| InChI Key | RTRQQBHATOEIAF-UUOKFMHZSA-N |
| Smiles | O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(C(N)=O)=C2N)[C@@H]1O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for AICAR
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 19.37 | 75 | |
| DMSO | 19.37 | 75 |
Preparing Stock Solutions for AICAR
The following data is based on the product molecular weight 258.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.75 mM | 5.16 mL | 25.82 mL | 51.63 mL |
| 3.75 mM | 1.03 mL | 5.16 mL | 10.33 mL |
| 7.5 mM | 0.52 mL | 2.58 mL | 5.16 mL |
| 37.5 mM | 0.1 mL | 0.52 mL | 1.03 mL |
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Product Datasheets for AICAR
References for AICAR
References are publications that support the biological activity of the product.
Giri et al (2004) 5-Aminoimidazole-4-carboxamide-1-β-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J.Neurosci. 24 479 PMID: 14724246
Kanazawa et al (2007) Adiponectin and AMP kinase activator stimulate proliferation, differentiation and mineralization of osteoblastic MC3T3-EI cells. BMC Cell Biol. 8 51 PMID: 18047638
Giri et al (2008) The role of AMPK in psychosine mediated effects on oligodendrocytes and astrocytes: Implication for Krabbe disease. J.Neurochem. 105 1820 PMID: 18248608
Wu et al (2011) AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells. Int.J.Artif.Organs. 34 1128 PMID: 22198598
If you know of a relevant reference for AICAR, please let us know.
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Keywords: AICAR, AICAR supplier, AMPK, activators, AMP-Activated, Protein, Kinases, Enzyme, Substrates, /, Activators, Osteogenic, Stem, Cells, Mesenchymal, 2840, Tocris Bioscience
20 Citations for AICAR
Citations are publications that use Tocris products. Selected citations for AICAR include:
Mo et al (2015) Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway. Nat Cell Biol 17 500 PMID: 25751140
Mohseni et al (2014) A genetic screen identifies an LKB1-MARK signalling axis controlling the Hippo-YAP pathway. PLoS One 16 108 PMID: 24362629
Seto et al (2013) Acute SimV. inhibits K ATP channels of porcine coronary artery myocytes. FASEB J 8 e66404 PMID: 23799098
Taub et al (2013) Distinct energy requirements for human memory CD4 T-cell homeostatic functions. BMC Cancer 27 342 PMID: 22972918
Yung et al (2013) Activation of AMPK inhibits cervical cancer cell growth through AKT/FOXO3a/FOXM1 signaling cascade. Stem Cells Transl Med 13 327 PMID: 23819460
Hutchins et al (2013) Calcium release-dependent actin flow in the leading process mediates axophilic migration. J Pharmacol Exp Ther 33 11361 PMID: 23843509
Bumpus and Johnson (2011) 5-Aminoimidazole-4-carboxyamide-ribonucleoside (AICAR)-stimulated hepatic expression of Cyp4a10, Cyp4a14, Cyp4a31, and other peroxisome proliferator-activated receptor α-responsive mouse genes is AICAR 5'-monophosphate-dependent and AMP-activated Proc Natl Acad Sci U S A 339 886 PMID: 21896918
Creighton et al (2011) Adenosine monophosphate-activated kinase alpha1 promotes endothelial barrier repair. FASEB J 25 3356 PMID: 21680893
Hashiguchi (2017) Direct versus indirect actions of ghrelin on hypothalamic NPY neurons. PLoS One 12 e0184261 PMID: 28877214
Abdulhag et al (2015) Mitochondrial complex IV deficiency, caused by mutated COX6B1, is associated with encephalomyopathy, hydrocephalus and cardiomyopathy. Eur J Hum Genet 23 159 PMID: 24781756
Mounier et al (2013) AMPKα1 regulates macrophage skewing at the time of resolution of inflammation during skeletal muscle regeneration. Cell Metab 18 251 PMID: 23931756
Fong et al (2013) Moderate excess of pyruvate augments osteoclastogenesis. Biol Open 2 387 PMID: 23616923
Kim et al (2016) Role of Inflammatory Signaling in the Differential Effects of Saturated and Poly-unsaturated Fatty Acids on Peripheral Circadian Clocks. EBioMedicine 7 100 PMID: 27322464
Hassan et al (2014) Reduced oxidative stress contributes to the lipid lowering effects of isoquercitrin in free fatty acids induced hepatocytes. Oxid Med Cell Longev 2014 313602 PMID: 25404990
Terry et al (2012) Human kinome profiling identifies a requirement for AMP-activated protein kinase during human cytomegalovirus infection. Nat Cell Biol 109 3071 PMID: 22315427
Jurmeister et al (2014) Transcriptomic analysis reveals inhibition of androgen receptor activity by AMPK in prostate cancer cells. Oncotarget 5 3785 PMID: 25003216
Abdou et al (2014) A cell-autonomous molecular cascade initiated by AMP-activated protein kinase represses steroidogenesis. Mol Cell Biol 34 4257 PMID: 25225331
Marinangeli et al (2018) AMP-Activated Protein Kinase Is Essential for the Maintenance of Energy Levels during Synaptic Activation. iScience 9 1 PMID: 30368077
Sen et al (2018) EWS-FLI1 reprograms the metabolism of Ewing sarcoma cells via positive regulation of glutamine import and serine-glycine biosynthesis. Mol Carcinog 57 1342 PMID: 29873416
Hu et al (2018) Berberine Attenuated Proliferation, Invasion and Migration by Targeting the AMPK/HNF4α/WNT5A Pathway in Gastric Carcinoma. Front Pharmacol 9 1150 PMID: 30405404
Do you know of a great paper that uses AICAR from Tocris? Please let us know.
Reviews for AICAR
Average Rating: 4 (Based on 1 Review.)
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good product, satisfied.
By
Kishor Pant on 05/22/2019
Assay Type: In Vitro
Species: Human
Cancer and normal cell lines treated with 2mM for different times and we noticed modulation in the AMPK and p53 expression.
Pathways for AICAR
