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Submit Review5(6)-SFX (Fluorescein), SE
Discontinued Product
6488 has been discontinued.
View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.
Description: Green fluorescent dye for the labeling of primary amines
Chemical Name: 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Purity: ≥90% (HPLC)
Biological Activity for 5(6)-SFX (Fluorescein), SE
5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm-1M-1.
Please see the protocol for further information on protein/antibody labeling and conjugation.
Optical Data for 5(6)-SFX (Fluorescein), SE
| Emission Color | Green |
|---|---|
| λabs | 494 nm |
| λem | 518 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Application | Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for 5(6)-SFX (Fluorescein), SE
| M. Wt | 586.55 |
| Formula | C31H26N2O10 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 114616-31-8 |
| PubChem ID | 122174028 |
| InChI Key | WAGYELYAQOYZFU-UHFFFAOYSA-N |
| Smiles | OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)OC(C5=C3C=CC(C(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)=C5)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)OC(C%11=C9C=C(C(NCCCCCC(ON%12C(CCC%12=O)=O)=O)=O)C=C%11)=O)C=C7 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for 5(6)-SFX (Fluorescein), SE
References for 5(6)-SFX (Fluorescein), SE
References are publications that support the biological activity of the product.
Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345
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Citations for 5(6)-SFX (Fluorescein), SE
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Protocols for 5(6)-SFX (Fluorescein), SE
The following protocol features additional information for the use of 5(6)-SFX (Fluorescein), SE (Cat. No. 6488).
Literature in this Area
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