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Discontinued Product
7665 has been discontinued.
View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.
Description: Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
Chemical Name: 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
Purity: ≥90% (HPLC)
Biological Activity for 5-TAMRA NHS
Key information: 5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.
Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M-1cm-1; A280 = 0.178.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Optical Data for 5-TAMRA NHS
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 546 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| pH Sensitivity | No |
| Correction Factor 280 | 0.178 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor® 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for 5-TAMRA NHS
| M. Wt | 527.52 |
| Formula | C29H25N3O7 |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 150810-68-7 |
| PubChem ID | 2762600 |
| InChI Key | VWFRSNKRTNUMET-UHFFFAOYSA-N |
| Smiles | CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for 5-TAMRA NHS
References for 5-TAMRA NHS
References are publications that support the biological activity of the product.
Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 20 1673 PMID: 19606815
Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716
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Keywords: 5-TAMRA, NHS, 5-TAMRA, NHS supplier, 5TAMRANHS, 5tamrase, fluorescent, dyes, DNA, RNA, amine, reactive, peptides, oligonucleotides, proteins, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, 7665, Tocris Bioscience
Citations for 5-TAMRA NHS
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Currently there are no citations for 5-TAMRA NHS.
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Protocols for 5-TAMRA NHS
The following protocol features additional information for the use of 5-TAMRA NHS (Cat. No. 7665).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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