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Submit ReviewBovine Serum Albumin
Description: BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
Purity: ≥98%
Biological Activity for Bovine Serum Albumin
Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.
Technical Data for Bovine Serum Albumin
| MW | ~66 kDa |
| Storage | Store at +4°C |
| Purity | ≥98% |
| CAS Number | 9048-46-8 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Bovine Serum Albumin
| Solubility | Soluble to 100 mg/ml in water |
Product Datasheets for Bovine Serum Albumin
References for Bovine Serum Albumin
References are publications that support the biological activity of the product.
If you know of a relevant reference for Bovine Serum Albumin, please let us know.
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9 Citations for Bovine Serum Albumin
Citations are publications that use Tocris products. Selected citations for Bovine Serum Albumin include:
Pamela J et al (2022) SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction. Hum Mol Genet 31 2693-2710 PMID: 35313342
P Michael et al (2022) The Circadian Clock in the Retinal Pigment Epithelium Controls the Diurnal Rhythm of Phagocytic Activity. Int J Mol Sci 23 PMID: 35628111
George P et al (2020) High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme. Sci Rep 10 22370 PMID: 33353972
David et al (2020) Dopamine 2 Receptor Signaling Controls the Daily Burst in Phagocytic Activity in the Mouse Retinal Pigment Epithelium. Invest Ophthalmol Vis Sci 61 10 PMID: 32396631
Serena et al (2023) Automated high-content imaging in iPSC-derived neuronal progenitors. SLAS Discov 28 42-51 PMID: 36610640
Glover et al (2023) The developmental basis of fingerprint pattern formation and variation Cell 186 1 PMID: 36764291
Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression, and cell proliferation. J Cell Sci PMID: 34031693
Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation. J Cell Sci 134 PMID: 34152388
Hanne C et al (2018) Comparison of two different culture conditions for derivation of early hiPSC. Cell Biol Int 42 1467-1473 PMID: 29603519
Do you know of a great paper that uses Bovine Serum Albumin from Tocris? Please let us know.
Reviews for Bovine Serum Albumin
Average Rating: 4.5 (Based on 4 Reviews.)
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BSA as blocking buffer.
By
Anonymous on 03/05/2023
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: A7R5 vSMC
5% blocking buffer for immunoflorescent staining.
5% BSA diluted in PBS. 30 min incubation with blocking buffer at RT
Blocking in Westerns.
By
Anna Højer Øster on 02/12/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Ngn3gfp
in the tc and western blotting
Very good.
By
Anonymous on 03/28/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: smooth muscle cell
We use this product for WB. It works very well.
Western blot.
By
Anonymous on 08/05/2018
Assay Type: In Vitro
Species: Mouse
Used to block PVDF membranes for WB. Perfect performance. Works better than milk