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Submit ReviewCell Counting Kit-8
Description: Cell viability and proliferation assay test solution
Alternative Names: CCK-8,WST-8
Biological Activity for Cell Counting Kit-8
Cell Counting Kit-8 is a viability and proliferation assay test solution. It is a supplied as a ready-to-use 1-bottle solution (5 mL; 500 tests) and enables the colorimetric determination of the number of viable cells in cell proliferation and cytotoxicity assays. Cell Counting Kit-8 utilizes a highly water-soluble tetrazolium salt (WST-8), which is reduced by cellular dehydrogenases to an orange formazan dye. The color change can be detected in the 430 - 490 nm range and Cell Counting Kit-8 exhibits improved sensitivity compared with other tetrazolium salt-based assays such as MTT, XTT or MTS. Can also be used in the detection of superoxide.
For more information on how to use Cell Counting Kit-8, please see our protocol.
Scientific Data
Cell Number Determination. HeLa cells 1x104 cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates. 10 μL Cell Counting Kit-8 or 10 μL MTT (Catalog # 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.
Cytotoxicity Assay. HeLa cells 1x104 cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates with staurosporine (Catalog # 1285). 10 μL Cell Counting Kit-8 or 10 μL MTT (Cat. No. 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.
Technical Data for Cell Counting Kit-8
| Storage | Store at +4°C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Cell Counting Kit-8
References for Cell Counting Kit-8
References are publications that support the biological activity of the product.
Ishiyama et al (1997) A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 44 1299 PMID: 18966866
Tominaga et al (1999) A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal.Commun. 36 47
Zhu et al (2020) Comparison of three analytical methods for superoxide produced by activated immune cells. J.Pharmacol.Toxicol.Methods 101 106637 PMID: 31678429
If you know of a relevant reference for Cell Counting Kit-8, please let us know.
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