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Cholesterol

Cat. No. 7945 Submit a Review Datasheet / COA / SDS
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Description: Component of lipid nanoparticles (LNPs); modulator of lipid bilayer fluidity
Chemical Name: (3β)-Cholest-5-en-3-ol
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Cholesterol

Cholesterol is a major sterol in all mammalian organisms; it is an essential component that makes up about 20-25% of the structural lipids of cell membranes. Cholesterol determines the permeability and fluidity characteristics of the cell membranes. Cholesterol is an endogenous ligand for estrogen-related receptor α (ERRα). It is also a precursor in several biosynthetic pathways, including steroid hormones and the active form of vitamin D. Cholesterol is a component of lipid nanoparticles (LNPs), and is important in modulating membrane stability, with the effect being context dependent. When combined with lipids that have low gel-liquid crystalline phase transition temperature (Tm), Cholesterol reduces membrane fluidity and increases bilayer thickness; when combined with high Tm lipids, it increases membrane fluidity and narrows the bilayer thickness.

For more information on how to prepare MC3 lipid nanoparticles for RNA delivery, please see our protocol.

Technical Data for Cholesterol

M. Wt 386.65
Formula C27H46O
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 57-88-5
PubChem ID 5997
InChI Key HVYWMOMLDIMFJA-DPAQBDIFSA-N
Smiles C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CCCC(C)C)C)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Cholesterol

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 19.33 50 with gentle warming
chloroform 38.66 100

Preparing Stock Solutions for Cholesterol

The following data is based on the product molecular weight 386.65. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.59 mL 12.93 mL 25.86 mL
5 mM 0.52 mL 2.59 mL 5.17 mL
10 mM 0.26 mL 1.29 mL 2.59 mL
50 mM 0.05 mL 0.26 mL 0.52 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References for Cholesterol

References are publications that support the biological activity of the product.

Tenchov et al (2021) Lipid nanoparticles - from liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano 15 16982 PMID: 34181394

Cheng et al (2018) Dendrimer-based lipid nanoparticles deliver therapeutic fah mRNA to normalize liver function and extend survival in a mouse model of hepatorenal tyrosinemia type I. Adv.Mater 30 e1805308 PMID: 30368954

Ghanbari et al (2020) Cholesterol as an endogenous ligand of ERRα promotes ERRα-mediated cellular proliferation and metabolic target gene expression in breast cancer cells. Cell 9 1765 PMID: 32717915

If you know of a relevant reference for Cholesterol, please let us know.

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Citations for Cholesterol

Citations are publications that use Tocris products.

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Protocols for Cholesterol

The following protocol features additional information for the use of Cholesterol (Cat. No. 7945).