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Submit ReviewCyanine 3, SE
Description: Orange fluorescent dye for labeling of amines; supplied as NHS ester
Chemical Name: 2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity: ≥95% (HPLC)
Biological Activity for Cyanine 3, SE
Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.
Optical Data for Cyanine 3, SE
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 550 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.09 |
| Application | Flow Cytometry |
| Alternative For | Alexa Fluor 546; DyLight 549 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for Cyanine 3, SE
| M. Wt | 727.84 |
| Formula | C35H41N3O10S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 146368-16-3 |
| PubChem ID | 91811030 |
| InChI Key | SOVMUUCAZOFIQP-UHFFFAOYSA-N |
| Smiles | CC[N+]1=C(C(C)(C)C2=C1C=CC(S(=O)([O-])=O)=C2)/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)(O)=O)=C5)=C5C/3(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Cyanine 3, SE
References for Cyanine 3, SE
References are publications that support the biological activity of the product.
Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407
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Keywords: Cyanine 3, SE, Cyanine 3, SE supplier, cyanine, NHS, ester, orange, fluorescent, dye, imaging, amines, labeling, Cy3, Cyanine, Dyes, (Cy, Dyes), Flow, Cytometry, 5435, Tocris Bioscience
Citations for Cyanine 3, SE
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Fluorescent probe production and application.
By
Vladimir Khayenko on 03/20/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cortical neurons
The Sulfo-Cyanine dye was conjugated to bioactive peptide that binds gephyrin. The labeling of gephyrin was done in Cortical neurons (DIV21) infected with lentivirus to express mEos2-Gephyrin.
Protocols for Cyanine 3, SE
The following protocol features additional information for the use of Cyanine 3, SE (Cat. No. 5435).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
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