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IPTG

Cat. No. 5509 Submit a Review Datasheet / COA / SDS
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Description: Used in cloning procedures with X-GAL
Chemical Name: 1-Methylethyl 1-thio-β-D-galactopyranoside
Purity: ≥99% (HPLC)
Datasheet
Citations
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for IPTG

IPTG is an used in combination with X-GAL (Cat. No. 5510) in cloning procedures requiring expression of β-galactosidase activity. Non-metabolizable analog of galactose.

This product has <0.1% dioxane content.

Technical Data for IPTG

M. Wt 238.3
Formula C9H18O5S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 367-93-1
PubChem ID 656894
InChI Key BPHPUYQFMNQIOC-NXRLNHOXSA-N
Smiles O[C@@H]1[C@H](O)[C@@H](O)[C@H](SC(C)C)O[C@@H]1CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IPTG

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 23.83 100

Preparing Stock Solutions for IPTG

The following data is based on the product molecular weight 238.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.2 mL 20.98 mL 41.96 mL
5 mM 0.84 mL 4.2 mL 8.39 mL
10 mM 0.42 mL 2.1 mL 4.2 mL
50 mM 0.08 mL 0.42 mL 0.84 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets for IPTG

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for IPTG

References are publications that support the biological activity of the product.

If you know of a relevant reference for IPTG, please let us know.

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Citations for IPTG

Citations are publications that use Tocris products.

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