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Description: Reagent used in the measurement of cell proliferation
Chemical Name: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Purity: ≥98%
Biological Activity for MTT
MTT is a reagent used in the measurement of cell proliferation.
Technical Data for MTT
| M. Wt | 414.32 |
| Formula | C18H16N5SBr |
| Storage | Store at +4°C |
| Purity | ≥98% |
| CAS Number | 298-93-1 |
| PubChem ID | 64965 |
| InChI Key | AZKSAVLVSZKNRD-UHFFFAOYSA-M |
| Smiles | CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for MTT
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| PBS | 5 | 13 |
Preparing Stock Solutions for MTT
The following data is based on the product molecular weight 414.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.13 mM | 18.57 mL | 92.83 mL | 185.66 mL |
| 0.65 mM | 3.71 mL | 18.57 mL | 37.13 mL |
| 1.3 mM | 1.86 mL | 9.28 mL | 18.57 mL |
| 6.5 mM | 0.37 mL | 1.86 mL | 3.71 mL |
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Product Datasheets for MTT
References for MTT
References are publications that support the biological activity of the product.
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Keywords: MTT, MTT supplier, MTT, measurement, cell, proliferation, Biochemicals, and, Molecular, Biology, 5224, Tocris Bioscience
5 Citations for MTT
Citations are publications that use Tocris products. Selected citations for MTT include:
Yusuke et al (2021) Osteoblasts derived from mouse mandible enhance tumor growth of prostate cancer more than osteoblasts derived from long bone. J Bone Oncol 26 100346 PMID: 33425674
Evan et al (2022) Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity. Biomolecules 12 PMID: 35454069
Xiao et al (2020) Analysis of Key Genes Regulating the Warburg Effect in Patients with Gastrointestinal Cancers and Selective Inhibition of This Metabolic Pathway in Liver Cancer Cells. Onco Targets Ther 13 7295-7304 PMID: 32801756
Jeffrey L et al (2023) Aldose reductase inhibition decelerates optic nerve degeneration by alleviating retinal microglia activation. Sci Rep 13 5592 PMID: 37019993
Aakrosh et al (2023) Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer Cells. Cancer Res Commun 3 592-606 PMID: 37077937
Do you know of a great paper that uses MTT from Tocris? Please let us know.
Reviews for MTT
Average Rating: 4.5 (Based on 2 Reviews.)
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Detection of apoptosis via cell proliferation assay MTT was carried out.
By
Anonymous on 02/11/2019
Species: Human
Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.
MTT as a tool to estimate cell proliferation.
By
Anonymous on 12/12/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Cardiomyocytes
HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.
