Home / Pharmacology / Enzymes / Kinases / CaM Kinase / Calmodulin-Dependent Kinase Inhibitors / NH 125

NH 125

Cat. No. 3439 5 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: CaM kinase III (eEF-2 kinase) inhibitor
Chemical Name: 1-Hexadecyl-2-methyl-3-(phenylmethyl)-1H-imidazolium iodide
Datasheet
Citations (5)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for NH 125

NH 125 is an eukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC50 = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC50 values are 0.7 - 4.8 μM) and blocks G1/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC50 = 6.6 μM) and in vivo.

Technical Data for NH 125

M. Wt 524.56
Formula C27H45IN2
Storage Desiccate at -20°C
CAS Number 278603-08-0
PubChem ID 10436839
InChI Key RVWOHCBHAGBLLT-UHFFFAOYSA-M
Smiles CC1=[N+](CCCCCCCCCCCCCCCC)C=CN1CC2=CC=CC=C2.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NH 125

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 52.46 100
ethanol 39.34 75

Preparing Stock Solutions for NH 125

The following data is based on the product molecular weight 524.56. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.91 mL 9.53 mL 19.06 mL
5 mM 0.38 mL 1.91 mL 3.81 mL
10 mM 0.19 mL 0.95 mL 1.91 mL
50 mM 0.04 mL 0.19 mL 0.38 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for NH 125

References are publications that support the biological activity of the product.

Yamamoto et al (2001) Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis. Biosci.Biotechnol.Biochem. 65 2306 PMID: 11758928

Arora et al (2003) Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines. Cancer Res. 63 6894 PMID: 14583488

Arora et al (2004) P-glycoprotein mediates resistance to histidine kinase inhibitors. Mol.Pharmacol. 66 460 PMID: 15322237

Chen (2011) 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor. J.Biol.Chem. 286 43951 PMID: 22020937

If you know of a relevant reference for NH 125, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Calmodulin-Dependent Kinase Inhibitors

Keywords: NH 125, NH 125 supplier, CaM, kinase, III, CAMK, eEF-2, inhibitors, inhibits, Histidine, protein, kinases, Calmodulin-Activated, calmodulin-dependent, Protein, NH125, Kinase, Other, Kinases, 3439, Tocris Bioscience

5 Citations for NH 125

Citations are publications that use Tocris products. Selected citations for NH 125 include:

Kim et al (2015) Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy. PLoS One 10 e0127640 PMID: 26039584

Xu et al (2017) Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity. Cell Res 27 882 PMID: 28429770

Francis et al (2013) Mechanistic insight into inhibition of two-component system signaling. Medchemcomm 4 269 PMID: 23336064

Cheng et al (2013) Integrated regulation of autophagy and apoptosis by EEF2K controls cellular fate and modulates the efficacy of curcumin and vel. against tumor cells. Autophagy 9 208 PMID: 23182879

Do you know of a great paper that uses NH 125 from Tocris? Please let us know.

Reviews for NH 125

There are currently no reviews for this product. Be the first to review NH 125 and earn rewards!

Have you used NH 125?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review