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Submit ReviewNutlin-3
Description: MDM2 antagonist; inhibits MDM2-p53 interaction
Chemical Name: (±)-4-[4,5-Bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxy-phenyl)-4,5-dihydro-imidazole-1-carbonyl]-piperazin-2-one
Purity: ≥98% (HPLC)
Biological Activity for Nutlin-3
Nutlin-3 is a MDM2 antagonist; inhibits the MDM2-p53 interaction (IC50 = 0.09 μM) and activates p53. Antiproliferative agent; induces apoptosis in cancer cells.
Licensing Information
Sold under license from F. Hoffmann-La Roche Ltd.
Technical Data for Nutlin-3
| M. Wt | 581.49 |
| Formula | C30H30Cl2N4O4 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 548472-68-0 |
| PubChem ID | 16755649 |
| InChI Key | BDUHCSBCVGXTJM-IZLXSDGUSA-N |
| Smiles | ClC1=CC=[C@@]([C@@H]3[C@@H](N(C(N5CC(NCC5)=O)=O)C(C4=CC=C(OC)C=C4OC(C)C)=N3)[C@]2=CC=C(Cl)C=C2)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nutlin-3
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 58.15 | 100 | |
| ethanol | 58.15 | 100 |
Preparing Stock Solutions for Nutlin-3
The following data is based on the product molecular weight 581.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.72 mL | 8.6 mL | 17.2 mL |
| 5 mM | 0.34 mL | 1.72 mL | 3.44 mL |
| 10 mM | 0.17 mL | 0.86 mL | 1.72 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.34 mL |
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Product Datasheets for Nutlin-3
References for Nutlin-3
References are publications that support the biological activity of the product.
Vassilev et al (2004) In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303 844 PMID: 14704432
If you know of a relevant reference for Nutlin-3, please let us know.
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Keywords: Nutlin-3, Nutlin-3 supplier, Nutlin3, p53, activator, MDM2, antagonist, antagonists, p53-MDM2, Ubiquitin, E3, Ligases, 3984, Tocris Bioscience
8 Citations for Nutlin-3
Citations are publications that use Tocris products. Selected citations for Nutlin-3 include:
Daniel P et al (2021) Replication stress promotes cell elimination by extrusion. Nature 593 591-596 PMID: 33953402
Yang et al (2016) A redox mechanism underlying nucleolar stress sensing by nucleophosmin. Nat Commun 7 13599 PMID: 27886181
Keeva et al (2018) Discovery and characterization of highly potent and selective allosteric USP7 inhibitors. Nat Chem Biol 14 118-125 PMID: 29200206
Alan R et al (2013) Small molecule induced reactivation of mutant p53 in cancer cells. Nucleic Acids Res 41 6034-44 PMID: 23630318
Gergely et al (2022) Comparison of Different Clinical Chemotherapeutical Agents' Toxicity and Cell Response on Mesenchymal Stem Cells and Cancer Cells. Cells 11 PMID: 36230904
Norlin et al (2018) The ATPase activity of Asna1/TRC40 is required for pancreatic progenitor cell survival. Development 145 PMID: 29180572
Bussey et al (2016) Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma. J Zhejiang Univ Sci B 5 1 PMID: 26754547
Peter et al (2020) Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway. Life Sci Alliance 3 PMID: 32651191
Do you know of a great paper that uses Nutlin-3 from Tocris? Please let us know.
Reviews for Nutlin-3
Average Rating: 5 (Based on 1 Review.)
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In vitro cell treatment using Nutlin (Cat. No. 3984).
By
Anonymous on 01/08/2018
Assay Type: In Vitro
Species: Human
It worked perfectly at 10microM concentration for 48 hours.
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