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Submit ReviewOcean Blue, SE
Description: Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Chemical Name: 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
Purity: ≥95% (HPLC)
Biological Activity for Ocean Blue, SE
Key information: Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Flow cytometry, fluorescence microscopy.
Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M-1cm-1; A280 correction factor = 0.15.
Please see the protocol for further information on protein/antibody labeling and conjugation.
Note: This product is typically prepared in DMSO.
Optical Data for Ocean Blue, SE
| Emission Color | Blue |
|---|---|
| Brightness | |
| λabs | 405 nm |
| λem | 455 nm |
| Extinction Coefficient (ε) | 36000 M-1cm-1 |
| Quantum Yield (φ) | 0.78 |
| Closest Laser line | 405 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.15 |
| Application | Flow Cytometry, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Ocean Blue, SE
| M. Wt | 339.2 |
| Formula | C14H7F2NO7 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 215868-33-0 |
| PubChem ID | 56927770 |
| InChI Key | NZYXABPVYJRICY-UHFFFAOYSA-N |
| Smiles | OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Ocean Blue, SE
References for Ocean Blue, SE
References are publications that support the biological activity of the product.
Sun et al (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 8 3107 PMID: 9873685
Brandtzaeg et al (1998) The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 216 49 PMID: 9760215
Haugland et al (2001) Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. PMID: 18228336
Cox et al (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 36 114 PMID: 14740493
If you know of a relevant reference for Ocean Blue, SE, please let us know.
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Protocols for Ocean Blue, SE
The following protocol features additional information for the use of Ocean Blue, SE (Cat. No. 6489).
Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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