Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewOG 488, SE
Discontinued Product
6257 has been discontinued.
View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.
Description: Green fluorescent dye; supplied as NHS ester
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
Biological Activity for OG 488, SE
OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.
Optical Data for OG 488, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 496 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 76,000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
| Correction Factor 280 | 0.12 |
| Application | Imaging, acidic organelle probe, flow, STED |
| Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for OG 488, SE
| M. Wt | 509.37 |
| Formula | C25H13F2NO9 |
| Storage | Store at -20°C |
| CAS Number | 198139-51-4 |
| PubChem ID | 58720805 |
| InChI Key | RPOLNGNGJOEMSR-UHFFFAOYSA-N |
| Smiles | OC1=C(F)C=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C(O)=O)=C(C=C4F)C(O5)=CC4=O)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for OG 488, SE
References for OG 488, SE
References are publications that support the biological activity of the product.
Vergene et al (1998) Phagosomal pH determination by dual fluorescence flow cytometry. Anal.Biochem. 255 127 PMID: 9448851
View Related Products by Target
Keywords: OG 488, SE, OG 488, SE supplier, OG488SE, green, fluorescent, dye, NHS, ester, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6257, Tocris Bioscience
Citations for OG 488, SE
Citations are publications that use Tocris products.
Currently there are no citations for OG 488, SE.
Reviews for OG 488, SE
There are currently no reviews for this product. Be the first to review OG 488, SE and earn rewards!
Have you used OG 488, SE?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Protocols for OG 488, SE
The following protocol features additional information for the use of OG 488, SE (Cat. No. 6257).
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals