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Submit ReviewpreQ1-azide
Description: preQ1 with azide handle for click chemistry
Chemical Name: N-[6-[[(2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl]amino]hexyl]-3-(2-azidoethoxy)propanamide
Purity: ≥95% (HPLC)
Biological Activity for preQ1-azide
preQ1-azide is a modified analog of the natural substrate prequeosine1 (preQ1). It contains an azide handle allowing attachment of additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. preQ1-azide is a toolbox compound for RNA-TAG (transglycosylation at guanosine).
Other RNA-TAG toolbox compounds include preQ1-biotin (Cat. No. 7804) and preQ1- alkyne (Cat. No. 7806)
Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.
Licensing Information
Sold under license from The Regents of the University of California.
Technical Data for preQ1-azide
| M. Wt | 419.49 |
| Formula | C18H29N9O3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | YYJIXWDJSKVSGF-UHFFFAOYSA-N |
| Smiles | O=C1C2=C(NC=C2CNCCCCCCNC(CCOCCN=[N+]=[N-])=O)N=C(N)N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for preQ1-azide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.95 | 100 | |
| ethanol | 20.97 | 50 |
Preparing Stock Solutions for preQ1-azide
The following data is based on the product molecular weight 419.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.38 mL | 11.92 mL | 23.84 mL |
| 5 mM | 0.48 mL | 2.38 mL | 4.77 mL |
| 10 mM | 0.24 mL | 1.19 mL | 2.38 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
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Product Datasheets for preQ1-azide
References for preQ1-azide
References are publications that support the biological activity of the product.
Zhang et al (2022) Site-specific and enzymatic cross-linking of sgRNA enables wavelength-selectable photoactivated control of CRISPR gene editing. J.Am.Chem.Soc. 144 4487 PMID: 35257575
If you know of a relevant reference for preQ1-azide, please let us know.
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Keywords: preQ1-azide, preQ1-azide supplier, preQ1, azide, handle, click, chemistry, photoactivatable, probes, RNA, TAG, RNATAG, CuAAC, copper-catalyzed, catalysed, Cu(I), Biochemicals, and, Molecular, Biology, 7833, Tocris Bioscience
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