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Submit ReviewTFAX 488, SE
Description: Green fluorescent dye; amine reactive
Chemical Name: 3,6-Diamino-9-[2-carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-4,5-disulfoxanthylium bislithium salt
Biological Activity for TFAX 488, SE
TFAX 488, SE is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.
Optical Data for TFAX 488, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 73,000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| pH Sensitivity | pH insensitive pH 4-10 |
| Application | flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED |
| Alternative For | Alexa Fluor® 488, AF 488, FITC, Cy2 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for TFAX 488, SE
| M. Wt | 643.41 |
| Formula | C25H15N3O13S2Li2 |
| Storage | Store at -20°C |
| CAS Number | 222164-96-7 |
| PubChem ID | 23543054 |
| InChI Key | FYCGGEVYPRTFDS-UHFFFAOYSA-L |
| Smiles | [NH2+]=C1C=CC2=C(C3=CC=C(C(ON4C(CCC4=O)=O)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)([O-])=O)=C5OC2=C1S(=O)([O-])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for TFAX 488, SE
References for TFAX 488, SE
References are publications that support the biological activity of the product.
Rossi et al (2012) Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes. J.Cell Sci. 125 4405 PMID: 22718347
Burnette et al (2011) Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc.Natl.Acad.Sci.USA. 108 21081 PMID: 22167805
van de Linde et al (2011) Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat.Protoc. 6 991 PMID: 21720313
Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID: 16141322
Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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