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Submit ReviewThiazovivin
Description: ROCK inhibitor; improves the efficiency of fibroblast reprogramming and induction of iPSCs
Chemical Name: N-Benzyl-[2-(pyrimidin-4-yl)amino]thiazole-4-carboxamide
Purity: ≥98% (HPLC)
Biological Activity for Thiazovivin
Thiazovivin is a selective, cell-permeable Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor (IC50 = 0.5 μM). Thiazovivin enhances the efficiency of fibroblast reprogramming to generate induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. No. 1614) and PD 0325901 (Cat. No. 4192). Thiazovivin improves the survival of human embryonic stem cells (hESCs) upon trypsinization and increases cell adhesion through the regulation of E-cadherin and significantly improves direct reprogramming efficiency. In combination with Valproic acid, sodium salt (Cat. No. 2815), Purmorphamine (Cat. No. 4551) and A 83-01 (Cat. No. 2939), Thiazovivin can be used to directly reprogram mouse embryonic fibroblasts into chemically induced neural stem cells.
Technical Data for Thiazovivin
| M. Wt | 311.36 |
| Formula | C15H13N5OS |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1226056-71-8 |
| PubChem ID | 46209426 |
| InChI Key | DOBKQCZBPPCLEG-UHFFFAOYSA-N |
| Smiles | O=C(NCC3=CC=CC=C3)C1=CSC(NC2=CC=NC=N2)=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Thiazovivin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.14 | 100 |
Preparing Stock Solutions for Thiazovivin
The following data is based on the product molecular weight 311.36. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.21 mL | 16.06 mL | 32.12 mL |
| 5 mM | 0.64 mL | 3.21 mL | 6.42 mL |
| 10 mM | 0.32 mL | 1.61 mL | 3.21 mL |
| 50 mM | 0.06 mL | 0.32 mL | 0.64 mL |
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Product Datasheets for Thiazovivin
References for Thiazovivin
References are publications that support the biological activity of the product.
Lin et al (2009) A chemical platform for improved induction of human iPSCs. Nat.Methods. 6 805 PMID: 19838168
Xu et al (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc.Natl.Acad.Sci. USA 107 8129 PMID: 20406903
Zheng et al (2016) A combination of small molecules directly reprograms mouse fibroblasts into neural stem cells. Biochem.Biophys.Res.Commun. 476 42 PMID: 27207831
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Keywords: Thiazovivin, Thiazovivin supplier, stem, cells, reprogramming, dedifferentiation, enhances, enhancers, induces, induced, pluripotent, ipscs, escs, neural, fibroblast, Stem, Cell, Reprogramming, Rho-kinases, Proliferation, Organoids, 3845, Tocris Bioscience
16 Citations for Thiazovivin
Citations are publications that use Tocris products. Selected citations for Thiazovivin include:
Kirsten et al (2021) Human physiomimetic model integrating microphysiological systems of the gut, liver, and brain for studies of neurodegenerative diseases. Sci Adv 7 PMID: 33514545
Douglas G et al (2021) Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices. STAR Protoc 2 100442 PMID: 33899023
Brown (2017) Live imaging reveals that the first division of differentiating human embryonic stem cells often yields asymmetric fates. Cell Rep 21 301 PMID: 29020617
Sevilla et al (2018) Derivation and characterization of the NIH registry human stem cell line NYSCF101 under defined feeder-free conditions. Stem Cell Res 29 197 PMID: 29730569
Caires-Júnior (2018) Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells. Nat Commun 9 475 PMID: 29396410
Jia-Jie et al (2022) Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency. Cell Rep 40 111240 PMID: 36001968
Lu et al (2022) Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nat Protoc 17 2699-2719 PMID: 35978039
Toshiaki et al (2022) Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species. Dev Growth Differ 64 325-341 PMID: 35841539
Mansa et al (2022) Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells. Cell Stem Cell 29 36-51.e6 PMID: 34856121
Tumelty et al (2018) Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein-Gremlin signaling pathways. J Biol Chem 293 3039 PMID: 29317497
Xu et al (2016) Wnt/β-catenin signaling promotes self-renewal and inhibits the primed state transition in naïve human embryonic stem cells. Proc Natl Acad Sci U S A 113 E6382 PMID: 27698112
Douglas A et al (2020) Clostridioides difficile-Associated Antibiotics Alter Human Mucosal Barrier Functions by Microbiome-Independent Mechanisms. Antimicrob Agents Chemother 64 PMID: 31988098
Mayana et al (2020) Differential gene expression elicited by ZIKV infection in trophoblasts from congenital Zika syndrome discordant twins. PLoS Negl Trop Dis 14 e0008424 PMID: 32745093
Omer et al (2020) Gut-Liver Physiomimetics Reveal Paradoxical Modulation of IBD-Related Inflammation by Short-Chain Fatty Acids. Cell Syst 10 223-239.e9 PMID: 32191873
Yang et al (2020) Xeno-Free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin-521. Curr Protoc Stem Cell Biol 52 e103 PMID: 31977148
Alexander R et al (2020) Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms. J Vac Sci Technol A 38 033205 PMID: 32255900
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.