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Submit Review5-BrdU
说明： Synthetic thymidine analog; used for cell proliferation assays
化学名： 5-Bromo-2-deoxyuridine
纯度： ≥99% (HPLC)
生物活性 for 5-BrdU
5-BrdU is a synthetic thymidine analog; incorporated into DNA during replication. Used in assays for cell proliferation. Replaces Oct-4 in transcription factor-mediated reprogramming of somatic cells and can be used in small molecule cocktail to generate ciPSCs.
化合物库 for 5-BrdU
5-BrdU is also offered as part of the Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。
技术数据 for 5-BrdU
| 分子量 | 307.1 |
| 公式 | C9H11BrN2O5 |
| 储存 | Store at -20°C |
| 纯度 | ≥99% (HPLC) |
| CAS Number | 59-14-3 |
| PubChem ID | 6035 |
| InChI Key | WOVKYSAHUYNSMH-RRKCRQDMSA-N |
| Smiles | O[C@H]1C[C@H](N2C=C(Br)C(NC2=O)=O)O[C@@H]1CO |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for 5-BrdU
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 15.36 | 50 | |
| DMSO | 30.71 | 100 |
制备储备液 for 5-BrdU
以下数据基于产品分子量 307.1。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.26 mL | 16.28 mL | 32.56 mL |
| 5 mM | 0.65 mL | 3.26 mL | 6.51 mL |
| 10 mM | 0.33 mL | 1.63 mL | 3.26 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.65 mL |
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产品说明书 for 5-BrdU
参考文献 for 5-BrdU
参考文献是支持产品生物活性的出版物。
Stacey and Hitomi (2008) Cell cycle studies basd upon quantitative image analysis. Cytometry A 73A 270 PMID: 18163464
Barker et al (2013) A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary. PLoS One 8 e63692 PMID: 23691086
Long et al (2015) Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells. Cell Res. 25 1171 PMID: 26251165
If you know of a relevant reference for 5-BrdU, please let us know.
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关键词: 5-BrdU, 5-BrdU supplier, 5-BrdU, DNA, synthesis, replication, cell, proliferation, assays, label, thymidine, analog, analogue, DNA,, RNA, and, Protein, Synthesis, Biochemicals, Molecular, Biology, Stem, Cell, Reprogramming, 5015, Tocris Bioscience
1 篇 5-BrdU 的引用文献
引用文献是使用了 Tocris 产品的出版物。 5-BrdU 的部分引用包括：
Muhammad Abid et al (2022) Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features. Cell Death Dis 13 670 PMID: 35915082
您是否知道使用了 Tocris 5-BrdU 的优秀论文？ 请告知我们.
5-BrdU 的评论
平均评分: 5 (Based on 1 Review.)
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Proliferation marker for studying neurogenesis.
By
Luiz Alexandre Magno on 07/17/2018
分析类型： In Vivo
种属： Mouse
细胞系/组织： Brain
I used BrdU to monitor cell proliferation in the mouse brain. Preparation of stock solutions of the drug is performed with warm (50ºC) sterile saline. It is advisable to keep the solution for 1h in the water bath at 50ºC with sonicator turned ON to complete solubilization. Aliquots can be kept at -80ºC until the use. The drug was diluted in saline 0.9% and injected i.p. (100 mg/kg). Intracardial perfusion was done 4 h later and BrdU was immunolabeled with an anti-BrdU antibody. The image displays that BrdU is present in the nucleus of the newly formed cells.
