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Ac-Cys-NHMe

Cat. No. 7936 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Building block for the lysine acylation using conjugating enzymes (LACE) technique
化学名： (2R)-2-(Acetylamino)-3-mercapto-N-methylpropanamide
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引用文献
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Ac-Cys-NHMe

Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery.

技术数据 for Ac-Cys-NHMe

分子量 176.23
公式 C6H12N2O2S
储存 Store at -20°C
CAS Number 10061-65-1
PubChem ID 11949287
InChI Key RAHKYMLIVZZMIH-YFKPBYRVSA-N
Smiles CNC([C@H](CS)NC(C)=O)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Ac-Cys-NHMe

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 17.62 100
water 17.62 100

制备储备液 for Ac-Cys-NHMe

以下数据基于产品分子量 176.23。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 5.67 mL 28.37 mL 56.74 mL
5 mM 1.13 mL 5.67 mL 11.35 mL
10 mM 0.57 mL 2.84 mL 5.67 mL
50 mM 0.11 mL 0.57 mL 1.13 mL

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产品说明书 for Ac-Cys-NHMe

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参考文献 for Ac-Cys-NHMe

参考文献是支持产品生物活性的出版物。

Levasseur et al (2022) Post-assembly modification of protein cages by Ubc9-mediated lysine acylation. Chembiochem 23 e202200332 PMID: 35951442

Hofmann et al (2020) Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins. Nat.Chem. 12 1008 PMID: 32929246

If you know of a relevant reference for Ac-Cys-NHMe, please let us know.

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关键词: Ac-Cys-NHMe, Ac-Cys-NHMe supplier, lysine, acylation, using, conjugating, enzymes, LACE, SUMO, conjugation, Ubc9, Unnatural, Amino, Acids, Biochemicals, and, Molecular, Biology, 7936, Tocris Bioscience

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