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Submit ReviewBDY 650-X, SE
Discontinued Product
5469 has been discontinued.
说明： Far-red BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
化学名： (T-4)-[2-[4-[2-[2-[([2,2'-Bi-1H-pyrrol]-5-yl-κN1)methylene]-2H-pyrrol-5-yl-κN]ethenyl]phenoxy]-N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]acetamidato]difluoroboron
纯度： ≥90% (HPLC)
生物活性 for BDY 650-X, SE
BDY 650-X, SE is a fluorescent far-red BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY 650-X makes it ideal for labeling lipids and cell membranes. BDY 650-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~646 nM; emission maximum ~660 nM.
For more information on how BDY 650-X, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY 650-X, SE
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 660 nm |
| Extinction Coefficient (ε) | 102000 M-1cm-1 |
| Quantum Yield (φ) | 0.52 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.04 |
| Application | Confocal fluorescence, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | BDY 630-X, SE; Cy5; Alexa Fluor 647 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for BDY 650-X, SE
| 分子量 | 643.44 |
| 公式 | C33H32BF2N5O6 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 235439-04-0 |
| PubChem ID | 25164046 |
| InChI Key | CDXXFTJLAKSQSR-JXMROGBWSA-N |
| Smiles | F[B-]1(F)[N+]2=C(C=CC2=CC3=CC=C(C4=CC=CN4)N13)/C=C/C5=CC=C(OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C=C5 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for BDY 650-X, SE
参考文献 for BDY 650-X, SE
参考文献是支持产品生物活性的出版物。
Yang et al (2013) A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking. Nat.Chem. 5 692 PMID: 23881501
关键词: BDY 650-X, SE, BDY 650-X, SE supplier, BDY650-X,SE, fluorescent, reagents, labeling, amines, bodipy, BDY, Dyes, 5469, Tocris Bioscience
1 篇 BDY 650-X, SE 的引用文献
引用文献是使用了 Tocris 产品的出版物。 BDY 650-X, SE 的部分引用包括：
Jane et al (2022) NR4A1 modulates intestinal smooth muscle cell phenotype and dampens inflammation-associated intestinal remodeling. FASEB J 36 e22609 PMID: 36250380
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals