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Submit ReviewFAM-DEALA-Hyp-YIPD
说明： Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
纯度： ≥95% (HPLC)
生物活性 for FAM-DEALA-Hyp-YIPD
FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.
Optical Data for FAM-DEALA-Hyp-YIPD
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence Polarization (FP) Displacement Assays |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for FAM-DEALA-Hyp-YIPD
| 分子量 | 1477.48 |
| 公式 | C71H84N10O25 |
| 序列 |
DEALAXYIPD (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp) |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | CWNCTVVEODBMBK-RHNKRQRVSA-N |
| Smiles | O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for FAM-DEALA-Hyp-YIPD
| 溶解性 | Soluble to 1 mg/ml in 0.01M PBS |
产品说明书 for FAM-DEALA-Hyp-YIPD
参考文献 for FAM-DEALA-Hyp-YIPD
参考文献是支持产品生物活性的出版物。
Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643
Lucas et al (2018) Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase. J.Med.Chem. 61 7387 PMID: 30040896
Roy et al (2019) SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 14 361 PMID: 30721025
Crews et al (2018) Identification and characterization of von Hippel-Lindau-recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-binding kinase 1. J.Med.Chem. 61 583 PMID: 28692295
Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556
If you know of a relevant reference for FAM-DEALA-Hyp-YIPD, please let us know.
按标靶查看相关产品
- Fluorescence Imaging
- Ubiquitin E3 Ligases
- Hypoxia Inducible Factors (HIF)
- Epigenetics
- Angiogenesis
- Ubiquitin/Proteasome System
- Enzyme Probes and Enzyme Substrates
- Transcription Factors
- Ligases
- TR-FRET and FP Assay Reagents
- Fluorescent Probes and Stains
- Ubiquitin E3 Ligase Peptides
- Hypoxia Inducible Factor (HIF) Peptides
- Enzyme Probes and Enzyme Substrate Peptides
- TR-FRET and FP Assay Reagents Peptides
关键词: FAM-DEALA-Hyp-YIPD, FAM-DEALA-Hyp-YIPD supplier, Fluorescent, HIF-1α, alfa, alpha, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, pathways, VHL, von, Hippel-Lindau, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, Ubiquitin, E3, Ligases, TR-FRET, FP, Assay, Reagents, 7287, Tocris Bioscience
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