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Submit ReviewFAM-DEALAHypYIPMDDDFQLRSF
Description: Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity: ≥95% (HPLC)
Biological Activity for FAM-DEALAHypYIPMDDDFQLRSF
FAM-DEALAHypYIPMDDDFQLRSF is a 5-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD = 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD (Cat. No. 7287).
Optical Data for FAM-DEALAHypYIPMDDDFQLRSF
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence Polarization (FP) Displacement Assays |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for FAM-DEALAHypYIPMDDDFQLRSF
| M. Wt | 2616.76 |
| Formula | C122H158N24O39S |
| Sequence |
DEALAXYIPMDDDFQLRSF (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp, Phe-19 = C-terminal amide) |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | BWRLQGDMCXAUCD-GMYGBYBYSA-N |
| Smiles | O=C(C1=CC(C(O2)=O)=C(C=C1)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N6[C@@H](CC[C@H]6O)C(N[C@@H](CC7=CC=C(C=C7)O)C(N[C@@H]([C@@H](C)CC)C(N8[C@@H](CCC8)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC9=CC=CC=C9)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%10=CC=CC=C%10)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(C(C=C%11)=CC(C%12%13C%14=CC=C(O)C=C%14OC%15=C%12C=CC(O)=C%15)=C%11C(O%13)=O)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N%16[C@@H](CC[C@H]%16O)C(N[C@@H](CC%17=CC=C(C=C%17)O)C(N[C@@H]([C@@H](C)CC)C(N%18[C@@H](CCC%18)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC%19=CC=CC=C%19)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%20=CC=CC=C%20)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for FAM-DEALAHypYIPMDDDFQLRSF
References for FAM-DEALAHypYIPMDDDFQLRSF
References are publications that support the biological activity of the product.
Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556
Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643
Crews et al (2018) Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. J.Med.Chem. 61 583 PMID: 28692295
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Keywords: FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALAHypYIPMDDDFQLRSF supplier, Fluorescent, HIF-1α, alpha, alfa, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, VHL, von, Hippel-Lindau, Ubiquitin, E3, Ligases, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, TR-FRET, FP, Assay, Reagents, 7452, Tocris Bioscience
Citations for FAM-DEALAHypYIPMDDDFQLRSF
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Currently there are no citations for FAM-DEALAHypYIPMDDDFQLRSF.
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