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Submit ReviewFAM-DEALAHypYIPMDDDFQLRSF
说明： Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
纯度： ≥95% (HPLC)
生物活性 for FAM-DEALAHypYIPMDDDFQLRSF
FAM-DEALAHypYIPMDDDFQLRSF is a 5-carboxyfluorescein (FAM) labeled HIF-1α peptide. It binds with high affinity to von Hippel-Lindau (VHL) protein (KD = 3 nM). Can be used to assess ligand binding to VHL in a direct fluorescence polarization (FP) displacement assay. Excitation/emission maxima (λ) = 485/520 nm. This product is a longer peptide version of FAM-DEALA-Hyp-YIPD (Cat. No. 7287).
Optical Data for FAM-DEALAHypYIPMDDDFQLRSF
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence Polarization (FP) Displacement Assays |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for FAM-DEALAHypYIPMDDDFQLRSF
| 分子量 | 2616.76 |
| 公式 | C122H158N24O39S |
| 序列 |
DEALAXYIPMDDDFQLRSF (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp, Phe-19 = C-terminal amide) |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | BWRLQGDMCXAUCD-GMYGBYBYSA-N |
| Smiles | O=C(C1=CC(C(O2)=O)=C(C=C1)C32C4=CC=C(O)C=C4OC5=C3C=CC(O)=C5)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N6[C@@H](CC[C@H]6O)C(N[C@@H](CC7=CC=C(C=C7)O)C(N[C@@H]([C@@H](C)CC)C(N8[C@@H](CCC8)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC9=CC=CC=C9)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%10=CC=CC=C%10)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.O=C(C(C=C%11)=CC(C%12%13C%14=CC=C(O)C=C%14OC%15=C%12C=CC(O)=C%15)=C%11C(O%13)=O)N[C@@H](CC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](C)C(N%16[C@@H](CC[C@H]%16O)C(N[C@@H](CC%17=CC=C(C=C%17)O)C(N[C@@H]([C@@H](C)CC)C(N%18[C@@H](CCC%18)C(N[C@@H](CCSC)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC%19=CC=CC=C%19)C(N[C@@H](CCC(N)=O)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC%20=CC=CC=C%20)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for FAM-DEALAHypYIPMDDDFQLRSF
参考文献 for FAM-DEALAHypYIPMDDDFQLRSF
参考文献是支持产品生物活性的出版物。
Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556
Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643
Crews et al (2018) Identification and Characterization of Von Hippel-Lindau-Recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-Binding Kinase 1. J.Med.Chem. 61 583 PMID: 28692295
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关键词: FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALAHypYIPMDDDFQLRSF supplier, Fluorescent, HIF-1α, alpha, alfa, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, VHL, von, Hippel-Lindau, Ubiquitin, E3, Ligases, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, TR-FRET, FP, Assay, Reagents, 7452, Tocris Bioscience
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