Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewFAM-DEALA-Hyp-YIPD
Description: Fluorescent HIF-1α peptide; can be used to evaluate the effect of VHL binding on degradation activity
Purity: ≥95% (HPLC)
Biological Activity for FAM-DEALA-Hyp-YIPD
FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide (Kd = 180-560 nM). Can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity. Excitation maximum = 485 nm, emission maximum = 535 nm. Longer peptide version, FAM-DEALAHypYIPMDDDFQLRSF (Cat. No. 7452), also available.
Optical Data for FAM-DEALA-Hyp-YIPD
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence Polarization (FP) Displacement Assays |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for FAM-DEALA-Hyp-YIPD
| M. Wt | 1477.48 |
| Formula | C71H84N10O25 |
| Sequence |
DEALAXYIPD (Modifications: Asp-1 = 5-FAM-Asp, X-6 = Hyp) |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | CWNCTVVEODBMBK-RHNKRQRVSA-N |
| Smiles | O=C([C@@H]1CCCN1C([C@H]([C@@H](CC)C)NC([C@H](CC2=CC=C(O)C=C2)NC([C@@H]3C[C@@H](O)CN3C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C4=CC(C(O5)=O)=C(C=C4)C65C7=CC=C(O)C=C7OC8=C6C=CC(O)=C8)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O.O=C([C@@H]9CCCN9C([C@H]([C@@H](CC)C)NC([C@H](CC%10=CC=C(O)C=C%10)NC([C@@H]%11C[C@@H](O)CN%11C([C@@H](NC([C@H](CC(C)C)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@@H](NC(C(C=C%12)=CC(C%13%14C%15=CC=C(O)C=C%15OC%16=C%13C=CC(O)=C%16)=C%12C(O%14)=O)=O)CC(O)=O)=O)=O)C)=O)=O)C)=O)=O)=O)=O)N[C@@H](CC(O)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FAM-DEALA-Hyp-YIPD
| Solubility | Soluble to 1 mg/ml in 0.01M PBS |
Product Datasheets for FAM-DEALA-Hyp-YIPD
References for FAM-DEALA-Hyp-YIPD
References are publications that support the biological activity of the product.
Buckley et al (2012) Targeting the von Hippel-Lindau E3 ubiquitin ligase using small molecules to disrupt the VHL/HIF-1a interaction. J.Am.Chem.Soc. 134 4465 PMID: 22369643
Lucas et al (2018) Surface probing by fragment-based screening and computational methods identifies ligandable pockets on the von Hippel-Lindau (VHL) E3 ubiquitin ligase. J.Med.Chem. 61 7387 PMID: 30040896
Roy et al (2019) SPR-measured kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem.Biol. 14 361 PMID: 30721025
Crews et al (2018) Identification and characterization of von Hippel-Lindau-recruiting Proteolysis Targeting Chimeras (PROTACs) of TANK-binding kinase 1. J.Med.Chem. 61 583 PMID: 28692295
Ahn et al (2009) HIF-1α peptide derivatives with modifications at the hydroxyproline residue as activators of HIF-1α. Bioorg.Med.Chem.Lett. 19 4403 PMID: 19515556
If you know of a relevant reference for FAM-DEALA-Hyp-YIPD, please let us know.
View Related Products by Target
- Fluorescence Imaging
- Ubiquitin E3 Ligases
- Hypoxia Inducible Factors (HIF)
- Epigenetics
- TR-FRET and FP Assay Reagents
- Angiogenesis
- Ubiquitin E3 Ligase Peptides
- Hypoxia Inducible Factor (HIF) Peptides
- Enzyme Probes and Enzyme Substrate Peptides
- TR-FRET and FP Assay Reagents Peptides
- Ubiquitin/Proteasome System
- Enzyme Probes and Enzyme Substrates
- Transcription Factors
- Ligases
- Fluorescent Probes and Stains
Keywords: FAM-DEALA-Hyp-YIPD, FAM-DEALA-Hyp-YIPD supplier, Fluorescent, HIF-1α, alfa, alpha, peptides, Fluorescence, polarization, (FP), displacement, assays, hypoxia, inducible, factor, HIF, pathways, VHL, von, Hippel-Lindau, Hypoxia, Inducible, Factors, Enzyme, Probes, and, Substrates, Ubiquitin, E3, Ligases, TR-FRET, FP, Assay, Reagents, 7287, Tocris Bioscience
Citations for FAM-DEALA-Hyp-YIPD
Citations are publications that use Tocris products.
Currently there are no citations for FAM-DEALA-Hyp-YIPD. Do you know of a great paper that uses FAM-DEALA-Hyp-YIPD from Tocris? Please let us know.
Reviews for FAM-DEALA-Hyp-YIPD
There are currently no reviews for this product. Be the first to review FAM-DEALA-Hyp-YIPD and earn rewards!
Have you used FAM-DEALA-Hyp-YIPD?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
TPD and Induced Proximity Research Product GuideUpdated
This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:
- Active Degraders
- TAG Degradation Platform
- Degrader Building Blocks
- Assays for Protein Degradation
- Induced Proximity Tools
Programmed Cell Death Poster
There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.
Targeted Protein Degradation Poster
Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia