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Submit Reviewtrans-ISRIB
说明： Integrated stress response (ISR) inhibitor
化学名： N,N'-trans-1,4-cyclohexanediylbis[2-(4-chlorophenoxy)acetamide]
纯度： ≥99% (HPLC)
生物活性 for trans-ISRIB
trans-ISRIB is a potent integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC50 = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.
技术数据 for trans-ISRIB
| 分子量 | 451.34 |
| 公式 | C22H24Cl2N2O4 |
| 储存 | Store at +4°C |
| 纯度 | ≥99% (HPLC) |
| CAS Number | 1597403-47-8 |
| PubChem ID | 1011240 |
| InChI Key | HJGMCDHQPXTGAV-UHFFFAOYSA-N |
| Smiles | O=C(COC1=CC=C(Cl)C=C1)N[C@@H]2CC[C@@H](NC(COC3=CC=C(Cl)C=C3)=O)CC2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for trans-ISRIB
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 4.51 | 10 温和加热 |
制备储备液 for trans-ISRIB
以下数据基于产品分子量 451.34。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 22.16 mL | 110.78 mL | 221.56 mL |
| 0.5 mM | 4.43 mL | 22.16 mL | 44.31 mL |
| 1 mM | 2.22 mL | 11.08 mL | 22.16 mL |
| 5 mM | 0.44 mL | 2.22 mL | 4.43 mL |
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产品说明书 for trans-ISRIB
参考文献 for trans-ISRIB
参考文献是支持产品生物活性的出版物。
Sidrauski et al (2013) Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2 e00498 PMID: 23741617
Sekine et al (2015) Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound. Science 348 1027 PMID: 25858979
Hinnebusch (2015) Cell biology. Blocking stress response for better memory? Science 348 967 PMID: 26023119
Chen et al (2021) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
If you know of a relevant reference for trans-ISRIB, please let us know.
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关键词: trans-ISRIB, trans-ISRIB supplier, Potent, inhibitors, inhibits, of, PERK, signaling, UPR, unfolded, protein, response, endoplasmic, reticulum, ER, stress, memory, consolidation, eIF2, alpha, kinases, ISR, integrated, ISRIB, stem, cell, maintenance, self, renewal, pluripotent, CEPT, differentiated, cryo, DNA,, RNA, and, Protein, Synthesis, Miscellaneous, Compounds, Other, stress/UPR, Stem, Cell, Proliferation, Cocktail, 5284, Tocris Bioscience
14 篇 trans-ISRIB 的引用文献
引用文献是使用了 Tocris 产品的出版物。 trans-ISRIB 的部分引用包括：
Jeffrey D et al (2022) Polyadenylated RNA and RNA-Binding Proteins Exhibit Unique Response to Hyperosmotic Stress. Front Cell Dev Biol 9 809859 PMID: 34970554
Jason A et al (2022) Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun 13 3244 PMID: 35688816
Jing et al (2022) Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR. Cell Rep 40 111092 PMID: 35858571
Chen et al (2019) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 18 528 PMID: 33941937
Gary W et al (2019) Adaptive Protein Translation by the Integrated Stress Response Maintains the Proliferative and Migratory Capacity of Lung Adenocarcinoma Cells. Mol Cancer Res 17 2343-2355 PMID: 31551255
Victor X et al (2019) The eIF2α Kinase GCN2 Modulates Period and Rhythmicity of the Circadian Clock by Translational Control of Atf4. Neuron 104 724-735.e6 PMID: 31522764
Jacques et al (2020) Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway. Nutrients 12 PMID: 33302552
Ryu et al (2023) Stress-free cell aggregation by using the CEPT cocktail enhances embryoid body and organoid fitness. Biofabrication 16 015016 PMID: 37972398
Lisa D et al (2023) A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas. Cancer Discov 13 766-795 PMID: 36576405
Tristan et al (2021) Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Rep. 16 3076 PMID: 34861164
Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep. 21 2895 PMID: 29212034
Bo et al (2021) SARS-CoV-2 infection induces beta cell transdifferentiation. Cell Metab 33 1577-1591.e7 PMID: 34081913
Viviana et al (2021) Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice. Science 372 PMID: 33888613
Peter et al (2018) Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. Mol Cell 71 629-636.e5 PMID: 30118681
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trans-ISRIB 的评论
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Inhibits integrated stress response in HEK293 cells.
By
mandi gandelman on 05/01/2019
分析类型： In Vitro
种属： Human
细胞系/组织： HEK293
Used trans-ISRIB to inhibit the integrated stress response (ISR) in HEK293 cells. Induced the ISR and added ISRIB (100 and 500nM) overnight. Evaluated p-eif2a (unchanged) and cleaved caspase 3 (increased in a dose-dependent manner with ISRIB.
High specific inhibitor.
By
Anonymous on 11/01/2017
分析类型： In Vitro
种属： Rat
细胞系/组织： cortical neurons
Toxicity assay. 500 nM concentration was used. Highly effective Integrated stress response (ISR) inhibitor. Works very well.
该领域的文献
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*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
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