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Submit ReviewBAPTA AM
Description: Cell-permeable Ca2+ chelator
Chemical Name: 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester)
Purity: ≥95% (HPLC)
Biological Activity for BAPTA AM
BAPTA AM is a selective calcium chelator that is the cell-permeable analog of BAPTA (Cat. No. 2786). Blocks hKv1.5, hKV11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM respectively). Displays antithrombotic activity in vitro.
F 127 (Cat. No. 6253) for the solubilization of BAPTA AM is also available.
Technical Data for BAPTA AM
| M. Wt | 764.68 |
| Formula | C34H40N2O18 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 126150-97-8 |
| PubChem ID | 2293 |
| InChI Key | YJIYWYAMZFVECX-UHFFFAOYSA-N |
| Smiles | O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for BAPTA AM
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.94 | 30 |
Preparing Stock Solutions for BAPTA AM
The following data is based on the product molecular weight 764.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.3 mM | 4.36 mL | 21.8 mL | 43.59 mL |
| 1.5 mM | 0.87 mL | 4.36 mL | 8.72 mL |
| 3 mM | 0.44 mL | 2.18 mL | 4.36 mL |
| 15 mM | 0.09 mL | 0.44 mL | 0.87 mL |
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Product Datasheets for BAPTA AM
References for BAPTA AM
References are publications that support the biological activity of the product.
Raspe et al (1989) Enhanced release of prostacyclin from quin 2-loaded endothelial cells. Eur.J.Pharmacol. 163 345 PMID: 2498111
Tang et al (2007) The membrane permeable calcium chelaotr BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem.Pharmacol. 74 1596 PMID: 17826747
Heilporn et al (1994) Synthesis of BAPTA-AM analogues capable of enhancing the vascuar production of prostacyclin. Bull.Soc.Chim.Belg. 103 309
If you know of a relevant reference for BAPTA AM, please let us know.
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Keywords: BAPTA AM, BAPTA AM supplier, Cell-permeable, Ca2+, chelator, hERG, KV11.1, hKv13, hKv15, channels, blockers, Calcium, Signaling, Signalling, Agents, General, Potassium, KV, Channels, voltage-gated, voltage-dependent, K+, BAPTAAM, Voltage-Gated, Biochemicals, and, Molecular, Biology, 2787, Tocris Bioscience
28 Citations for BAPTA AM
Citations are publications that use Tocris products. Selected citations for BAPTA AM include:
Alvarez-Arce (2017) Thrombin-induced calpain activation promotes protease-activated receptor 1 internalization. Int J Cell Biol 2017 1908310 PMID: 29250115
Barbaro (2017) Dendritic cell amiloride-sensitive channels mediate sodium-induced inflammation and hypertension. Cell Rep 21 1009 PMID: 29069584
Gong (2017) ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences Cell 169 286 PMID: 28388412
Vallabhapurapu et al (2015) Variation in human cancer cell external phosphatidylserine is regulated by flippase activity and intracellular calcium. Oncotarget 6 34375 PMID: 26462157
Pal et al (2013) Divergent β-arrestin-dependent signaling events are dependent upon sequences within G-protein-coupled receptor C termini. J Biol Chem 288 3265 PMID: 23235155
Lee et al (2012) Mycobacterium abscessus activates the NLRP3 inflammasome via Dectin-1-Syk and p62/SQSTM1. Immunol Cell Biol 90 601 PMID: 21876553
Miteva et al (2019) Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies. J Cell Biol PMID: 31092558
Markovics et al (2019) Nicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA2 ). J Cell Mol Med 23 6203 PMID: 31273921
Gray and Golowasch (2016) Voltage Dependence of a Neuromodulator-Activated Ionic Current. Eneuro 3 PMID: 27257619
Song et al (2018) Selective Role of RGS9-2 in Regulating Retrograde Synaptic Signaling of Indirect Pathway Medium Spiny Neurons in Dorsal Striatum. J Neurosci 38 7120 PMID: 30006367
Ahmed (2016) Effects of novel muscarinic M3 receptor ligand C1213 in pulmonary arterial hypertension models. Physiological Reports 4 e13069 PMID: 28039410
Kajiya et al (2012) Muscarinic type 3 receptor induces cytoprotective signaling in salivary gland cells through epidermal growth factor receptor transactivation. J Clin Biochem Nutr 82 115 PMID: 22511543
Lee et al (2012) The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. J Clin Invest 492 123 PMID: 23143333
Draganov et al (2015) Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via iverm. induces a non-apoptotic and inflammatory form of cancer cell death. Nature 5 16222 PMID: 26552848
Leoni et al (2015) Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. Sci Rep 125 1215 PMID: 25664854
Chen et al (2015) Anti-inflammatory effects of Perilla frutescens in activated human neutrophils through two independent pathways: Src family kinases and Calcium. BMC Complement Altern Med 5 18204 PMID: 26659126
Ahn et al (2015) Neuroprotection and spatial memory enhancement of four herbal mixture extract in HT22 hippocampal cells and a mouse model of focal cerebral ischemia. J Biol Chem 15 202 PMID: 26122524
Pecze et al (2015) Routes of Ca2+ Shuttling during Ca2+ Oscillations: FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS. MBio 290 28214 PMID: 26396196
Azab et al (2015) Binding of αherpesvirus Glycoprotein H to Surface α4β1-Integrins Activates Calcium-Signaling Pathways and Induces Phosphatidylserine Exposure on the Plasma Membrane. J Biol Chem 6 PMID: 26489864
Wang et al (2015) MFN2 couples glutamate excitotoxicity and mitochondrial dysfunction in motor neurons. Mol Pharmacol 290 168 PMID: 25416777
Campwala et al (2014) P2Y6 receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cells. J Cell Sci 127 4964 PMID: 25271060
Pandya and Pillai (2014) TrkB interacts with ErbB4 and regulates NRG1-induced NR2B phosphorylation in cortical neurons before synaptogenesis. Sci Rep 12 47 PMID: 25052836
Florea et al (2017) Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma. Oncotarget 8 22876 PMID: 28206967
Zhang et al (2017) Protein kinase D at the Golgi controls NLRP3 inflammasome activation. J Exp Med 214 2671 PMID: 28716882
Díaz et al (2015) Biomechanical forces promote blood development through prostaglandin E2 and the cAMP-PKA signaling axis. J Exp Med 212 665 PMID: 25870199
Morais et al (2015) L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and Necrosis. PLoS One 10 e0132569 PMID: 26193352
Pozner et al (2015) Intracellular calcium dynamics in cortical microglia responding to focal laser injury in the PC::G5-tdT reporter mouse. Front Mol Neurosci 8 12 PMID: 26005403
Jantarajit et al (2017) CFTR-mediated anion secretion across intestinal epithelium-like Caco-2 monolayer under PTH stimulation is dependent on intermediate conductance K+ channels. Am J Physiol Cell Physiol 313 C118 PMID: 28490422
Do you know of a great paper that uses BAPTA AM from Tocris? Please let us know.
Reviews for BAPTA AM
Average Rating: 5 (Based on 1 Review.)
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BAPTA AM used to induce apoptosis.
By
Anonymous on 01/24/2019
Species: Human
BAPTA AM being a chelator has been used on our lab multiple times to induce apoptosis in cell cultures.Works great
