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Submit ReviewWortmannin
Description: Potent, irreversible inhibitor of PI 3-kinase. Also inhibitor of PLK1
Alternative Names: SL-2052
Chemical Name: (1S,6bR,9aS,11R,11bR) 11-(Acetyloxy)-1,6b,7,8,9a,10,11,11b-octahydro-1-(methoxymethyl)-9a,11b-dimethyl-3H-furo[4,3,2-de]indeno[4,5,-h]-2-h]-2-benzopyran-3,6,9-trione
Purity: ≥98% (HPLC)
Biological Activity for Wortmannin
Wortmannin is a potent, selective, cell-permeable and irreversible inhibitor of phosphatidylinositol 3-kinase (PI 3-kinase) (IC50 = 2 - 4 nM). Also potently inhibits polo-like kinase 1 (PLK1) (IC50 = 5.8 nM).
Technical Data for Wortmannin
| M. Wt | 428.44 |
| Formula | C23H24O8 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 19545-26-7 |
| PubChem ID | 3003565 |
| InChI Key | QDLHCMPXEPAAMD-ZGSWIPFCSA-N |
| Smiles | O=C(C4=CO5)O[C@H](COC)[C@@]1(C)C4=C5C(C2=C1[C@H](OC(C)=O)C[C@@]3(C)C2CCC3=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Wortmannin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 21.42 | 50 |
Preparing Stock Solutions for Wortmannin
The following data is based on the product molecular weight 428.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.67 mL | 23.34 mL | 46.68 mL |
| 2.5 mM | 0.93 mL | 4.67 mL | 9.34 mL |
| 5 mM | 0.47 mL | 2.33 mL | 4.67 mL |
| 25 mM | 0.09 mL | 0.47 mL | 0.93 mL |
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Product Datasheets for Wortmannin
References for Wortmannin
References are publications that support the biological activity of the product.
Arcaro and Wymann (1993) Wortmannin is a potent phophatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses. Biochem.J. 296 297 PMID: 8257416
Liu et al (2005) Wortmannin, a widely used phosphoinositide 3-kinase inhibitor, also potently inhibits mammalian polo-like kinase. Chem.Biol. 12 99 PMID: 15664519
Powis et al (1994) Wortmannin, a potent and selective inhibitor of phosphatidylinositol-3-kinase. Cancer Res. 54 2419 PMID: 8162590
Schultz et al (1995) In vitro and in vivo antitumor activity of the phosphatidylinositol-3-kinase inhibitor wortmannin. Anticancer Res. 15 1135 PMID: 7653991
If you know of a relevant reference for Wortmannin, please let us know.
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Keywords: Wortmannin, Wortmannin supplier, Potent, inhibitors, inhibits, PLK1, PI, 3-kinase, irreversible, Polo-like, Kinases, PI3K, Mitosis, Phosphoinositide, SL2052, vps34, autophagy, SL-2052, Inositol, Lipids, 3-Kinase, Kinase, Autophagy, 1232, Tocris Bioscience
63 Citations for Wortmannin
Citations are publications that use Tocris products. Selected citations for Wortmannin include:
Royal et al (2017) Phosphatidylinositol-4,5-bisphosphate is required for KCNQ1/KCNE1 channel function but not anterograde trafficking. PLoS One 12 e0186293 PMID: 29020060
Villar-Pazos et al (2017) Molecular mechanisms involved in the non-monotonic effect of bisphenol-a on Ca2+ entry in mouse pancreatic β-cells. Sci Rep 7 11770 PMID: 28924161
Medrihan et al (2017) Initiation of Behavioral Response to Antidepressants by Cholecystokinin Neurons of the Dentate Gyrus. Neuron 95 564 PMID: 28735749
Ledonne et al (2015) Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons. Mol Psychiatry 20 959 PMID: 25266126
Liu et al (2009) Inhibitory effect of ETB receptor on Na(+)-K(+) ATPase activity by extracellular Ca(2+) entry and Ca(2+) release from the endoplasmic reticulum in renal proximal tubule cells. Hypertens Res 32 846 PMID: 19662022
Roselli et al (2009) Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways. PLoS One 4 e6011 PMID: 19547699
Garcia et al (2009) c-jun controls the ability of IL-12 to induce IL-10 production from human memory CD4+ T cells. J Immunol 183 4475 PMID: 19734233
Grodzki et al (2009) FcεRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation. Exp Neurol 46 2539 PMID: 19540596
Accardi et al (2015) α6-Containing GABAA Receptors Are the Principal Mediators of Inhibitory Synapse Strengthening by Ins in Cerebellar Granule Cells. J Neurosci 35 9676 PMID: 26134650
Rogers and Gahring (2015) Upregulation of Nicotinic Acetylcholine Receptor α4+β2 through a Ligand-Independent PI3Kβ Mechanism That Is Enhanced by TNFα and the Jak2/p38Mapk Pathways. Front Pharmacol 10 e0143319 PMID: 26619345
Mäki-Jouppila et al (2014) Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na. Nat Commun 13 1054 PMID: 24748653
Takada et al (2014) Mechanosensitive ATP release from hemichannels and Ca2+ influx through TRPC6 accelerate wound closure in keratinocytes. J Cell Sci 127 4159 PMID: 25097230
Grassin-Delyle et al (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res 14 134 PMID: 24266887
Cheshenko et al (2013) HSV activates Akt to trigger calcium release and promote viral entry: novel candidate target for treatment and suppression. FASEB J 27 2584 PMID: 23507869
McAlpine et al (2013) Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy 9 361 PMID: 23291478
Mohan et al (2013) Combination of LC3 shRNA plasmid transfection and genistein treatment inhibited autophagy and increased apoptosis in malignant neuroblastoma in cell culture and animal models. PLoS One 8 e78958 PMID: 24205354
Roselli et al (2011) CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton. PLoS One 6 e23097 PMID: 21829588
Sidhar and Giri (2017) Induction of Bex genes by curcumin is associated with apoptosis and activation of p53 in N2a neuroblastoma cells. Sci Rep 7 41420 PMID: 28145533
Nelson et al (2008) Temporal profiling of changes in phosphatidylinositol 4,5-bisphosphate, inositol 1,4,5-trisphosphate and diacylglycerol allows comprehensive analysis of phospholipase C-initiated signalling in single neurons. J Neurochem 107 602 PMID: 18665913
Garcia et al (2008) Antigenic experience dictates functional role of glycogen synthase kinase-3 in human CD4+ T cell responses. J Immunol 181 8363 PMID: 19050253
Martín-Segura et al (2019) Age-associated cholesterol reduction triggers brain Ins. resistance by facilitating ligand-independent receptor activation and pathway desensitization. Aging Cell 18 e12932 PMID: 30884121
Wijerathne et al (2019) Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner. Korean J Physiol Pharmacol 23 381 PMID: 31496875
Li et al (2016) Progesterone receptor membrane component-1 regulates hepcidin biosynthesis. J Clin Invest 126 389 PMID: 26657863
Huang et al (2010) Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor. J Neurosci 30 6080 PMID: 20427666
Heberle et al (2019) The PI3K and MAPK/p38 pathways control stress granule assembly in a hierarchical manner. Life Sci Alliance 2 PMID: 30923191
Olianas et al (2016) The GABAB positive allosteric modulators CGP7930 and GS39783 stimulate ERK1/2 signalling in cellslacking functional GABAB receptors European Journal of Pharmacology 794 135 PMID: 27876620
Ruisanchez et al (2014) Lysophosphatidic acid induces vasodilation mediated by LPA1 receptors, phospholipase C, and endothelial nitric oxide synthase. FASEB J 28 880 PMID: 24249637
Nazarians-Armavil et al (2013) Cellular Ins resistance disrupts leptin-mediated control of neuronal signaling and transcription. Mol Endocrinol 27 990 PMID: 23579487
Faria et al (2013) Melatonin acts through MT1/MT2 receptors to activate hypothalamic Akt and suppress hepatic gluconeogenesis in rats. Am J Physiol Endocrinol Metab 305 E230 PMID: 23695212
Villers et al (2012) Long-lasting LTP requires neither repeated trains for its induction nor protein synthesis for its development. PLoS One 7 e40823 PMID: 22792408
Chen et al (2012) The class I PI3K/Akt pathway is critical for cancer cell survival in dogs and offers an opportunity for therapeutic intervention. BMC Vet Res 8 73 PMID: 22647622
Khurana et al (2012) An essential role for modulation of hyperpolarization-activated current in the development of binaural temporal precision. J Neurosci 32 2814 PMID: 22357864
Bianca et al (2012) Endogenous urotensin II selectively modulates erectile function through eNOS. PLoS One 7 e31019 PMID: 22319601
Liu et al (2010) FRP inhibits ox-LDL-induced endothelial cell apoptosis through an Akt-NF-{kappa}B-Bcl-2 pathway and inhibits endothelial cell apoptosis in an apoE-knockout mouse model. Am J Physiol Endocrinol Metab 299 E351 PMID: 20530739
Ahmed (2016) Effects of novel muscarinic M3 receptor ligand C1213 in pulmonary arterial hypertension models. Physiological Reports 4 e13069 PMID: 28039410
Yao et al (2015) Endothelial Rac1 is essential for hematogenous metastasis to the lung. Sci Rep 6 17501 PMID: 25991673
Jiang et al (2015) Stimulation of EphB2 attenuates tau phosphorylation through PI3K/Akt-mediated inactivation of glycogen synthase kinase-3β. Mol Cancer 5 11765 PMID: 26119563
Koutsioumpa et al (2015) Receptor protein tyrosine phosphatase β/ζ is a functional binding partner for vascular endothelial growth factor. Oncotarget 14 19 PMID: 25644401
Sahay et al (2015) The LPA1/ZEB1/miR-21-activation pathway regulates metastasis in basal breast cancer. PLoS One 6 20604 PMID: 26098771
Collar et al (2018) The homozygous CX3CR1-M280 mutation impairs human monocyte survival. JCI Insight 3 PMID: 29415879
Zhang et al (2018) Integrin CD11b negatively regulates Mincle-induced signaling via the Lyn-SIRPα-SHP1 complex. Exp Mol Med 50 e439 PMID: 29400702
Lee et al (2016) Mincle-mediated translational regulation is required for strong nitric oxide production and inflammation resolution. Oncotarget 7 11322 PMID: 27089465
Maugeri et al (2016) PACAP and VIP Inhibit the Invasiveness of Glioblastoma Cells Exposed to Hypoxia through the Regulation of HIFs and EGFR Expression. Oncotarget 7 139 PMID: 27303300
Wilson et al (2016) SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination. Sci Rep 6 17735 PMID: 26787558
Yin et al (2012) TNF-α triggers rapid membrane insertion of Ca(2+) permeable AMPA receptors into adult motor neurons and enhances their susceptibility to slow excitotoxic injury. J Cell Sci 238 93 PMID: 22921461
Jiang and Zsombok (2014) Regulation of neurons in the dorsal motor nucleus of the vagus by SIRT1. Front Neurosci 7 270 PMID: 24454277
Mitrugno et al (2014) A novel and essential role for FcγRIIa in cancer cell-induced platelet activation. Blood 123 249 PMID: 24258815
Siddoway et al (2013) An essential role for inhibitor-2 regulation of protein phosphatase-1 in synaptic scaling. J Pharmacol Exp Ther 33 11206 PMID: 23825423
Choi et al (2013) EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-κB activity. J Virol 4 2383 PMID: 24280371
Stefanidou et al (2013) Herpes simplex virus 2 (HSV-2) prevents dendritic cell maturation, induces apoptosis, and triggers release of proinflammatory cytokines: potential links to HSV-HIV synergy. Mol Immunol 87 1443 PMID: 23152529
Venkateswarlu et al (2007) PI-3-kinase-dependent membrane recruitment of centaurin-α2 is essential for its effect on ARF6-mediated actin cytoskeleton reorganisation. J Neurosci 120 792 PMID: 17284522
O'Riordan et al (2006) Regulation of nuclear factor κB in the hippocampus by group I metabotropic glutamate receptors. J Neurosci 26 4870 PMID: 16672661
Bianca et al (2006) Sphingosine 1-phosphate induces endothelial nitric-oxide synthase activation through phosphorylation in human corpus cavernosum. J Cell Biochem 316 703 PMID: 16234413
Qiu et al (2006) Differential reelin-induced enhancement of NMDA and AMPA receptor activity in the adult hippocampus. Mol Cancer Ther 26 12943 PMID: 17167084
Lim et al (2008) The novel adipocytokine visfatin exerts direct cardioprotective effects. J Cell Mol Med 12 1395 PMID: 18400051
Coba et al (2008) Kinase networks integrate profiles of N-MthD.-aspartate receptor-mediated gene expression in hippocampus. J Biol Chem 283 34101 PMID: 18815127
Rojas et al (2007) Protein kinase C dependent inhibition of the heteromeric Kir4.1-Kir5.1 channel. Biochim Biophys Acta 1768 2030 PMID: 17585871
Chung et al (2007) IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4. J Endocrinol 194 77 PMID: 17592023
Lin et al (2007) GSK-3beta acts downstream of PP2A and the PI 3-kinase-Akt pathway, and upstream of caspase-2 in ceramide-induced mitochondrial apoptosis. J Cell Sci 120 2935 PMID: 17666435
Alonso-Magdalena et al Pancreatic Ins content regulation by the estrogen receptor ER alpha. PLoS One 3 e2069 PMID: 18446233
Searl and Silinsky (2005) LY 294002 inhibits adenosine receptor activation by a mechanism independent of effects on PI-3 kinase or casein kinase II. Purinergic Signal 1 389 PMID: 18404524
Schlemper et al (2005) Mechanisms underlying the relaxation response induced by bradykinin in the epithelium-intact guinea-pig trachea in vitro. Br J Pharmacol 145 740 PMID: 15852038
Tokuoka and Goda (2006) Myosin light chain kinase is not a regulator of synaptic vesicle trafficking during repetitive exocytosis in cultured hippocampal neurons. J Neurosci 26 11606 PMID: 17093082
Do you know of a great paper that uses Wortmannin from Tocris? Please let us know.
Reviews for Wortmannin
Average Rating: 4.5 (Based on 2 Reviews.)
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Antidepressantsby Cholecystokinin Neurons of the Dentate Gyrus.
By
Anonymous on 02/03/2020
Assay Type: In Vivo
Species: Mouse
10nM Wortmannin
Inhibition of autophagy before immunocytochemistry in human fibroblasts.
By
tom stoker on 09/17/2018
Assay Type: In Vitro
Species: Human
Treatment of fibroblasts for 4 hours prior to staining for LC3 and P62.
