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Submit ReviewBDY FL-X, SE
Discontinued Product
5620 has been discontinued.
说明： Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
化学名： (T-4)-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-&N)methyl]-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
纯度： ≥90% (HPLC)
生物活性 for BDY FL-X, SE
BDY FL-X, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to FITC and TFAX 488 dyes. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY FL-X, SE
| Emission Color | Green |
|---|---|
| λabs | 504 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 85000 M-1cm-1 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | FAM; Alexa Fluor 488; DyLight 488; Cy2 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for BDY FL-X, SE
| 分子量 | 502.32 |
| 公式 | C24H29BF2N4O5 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 217190-09-5 |
| PubChem ID | 13007361 |
| InChI Key | AWACOFBGFAKYPF-UHFFFAOYSA-N |
| Smiles | CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=CC3=C2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for BDY FL-X, SE
参考文献 for BDY FL-X, SE
参考文献是支持产品生物活性的出版物。
Baker et al (2010) Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands. Br.J.Pharmacol. 159 772 PMID: 20105183
关键词: BDY FL-X, SE, BDY FL-X, SE supplier, BDYFL-X,SE, fluorescent, reagents, labeling, amines, BDY, Dyes, 5620, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals