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Y-27632 dihydrochloride
说明： Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
别名： y27632,Y-27632 2HCl,ROCK inhibitor,ROCK1 inhibitor
化学名： trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride
纯度： ≥98% (HPLC)
生物活性 for Y-27632 dihydrochloride
Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR 99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Also used in a protocol to generate brain organoids from human iPSCs. Cell-permeable, orally bioavailable and active in vivo.
Y-27632 in solution and Y-27632 synthesized to cGMP guidelines also available.
For more information about how Y-27632 dihydrochloride may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts.
Scientific Data
Application of Y-27632 in MESP1+ Mesoderm. ROCK inhibition by Y-27632 (1 μM) promotes skeletal myogenic differentiation of MESP1+ mesoderm, as shown by immunostaining for Myosin Heavy Chain+ (Green) and Myogenin (Red), and counterstaining with DAPI (Catalog # 5748; Blue, Tocris). Data courtesy of Dr Sunny Chan and Dr Michael Kyba, University of Minnesota.
化合物库 for Y-27632 dihydrochloride
Y-27632 dihydrochloride is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。
技术数据 for Y-27632 dihydrochloride
| 分子量 | 320.26 |
| 公式 | C14H21N3O.2HCl |
| 储存 | Desiccate at RT |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 129830-38-2 |
| PubChem ID | 14801906 |
| InChI Key | IDDDVXIUIXWAGJ-LJDSMOQUSA-N |
| Smiles | N[C@H](C)[C@@]1([H])CC[C@H](C(NC2=CC=NC=C2)=O)CC1.Cl.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Y-27632 dihydrochloride
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 32.03 | 100 | |
| DMSO | 32.03 | 100 |
制备储备液 for Y-27632 dihydrochloride
以下数据基于产品分子量 320.26。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.22 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.24 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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产品说明书 for Y-27632 dihydrochloride
参考文献 for Y-27632 dihydrochloride
参考文献是支持产品生物活性的出版物。
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关键词: Y-27632 dihydrochloride, Y-27632 dihydrochloride supplier, Selective, p160, ROCK, inhibitors, inhibits, Rho-Kinase, Rho-Associated, Coiled-Coil, Kinases, ROK, stem, cells, organoids, induced, pluripotent, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell, freezing, media, 2HCl, y27632, Y-27632, inhibitor, Rho-kinases, Stem, Cell, Proliferation, Organoids, Reprogramming, Neural, Cells, 1254, Tocris Bioscience
252 篇 Y-27632 dihydrochloride 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Y-27632 dihydrochloride 的部分引用包括：
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Y-27632 dihydrochloride 的评论
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Have you used Y-27632 dihydrochloride?
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Consistent performance.
By
Ted Mason on 08/22/2023
分析类型： In Vitro
种属： Human
ROCKi has consistently performed in iPSC cell culture work. Well packaged vials are resuspended and aliquoted for -20 storage. Cells passaged or fed with ROCKi media have shown good viability. This is a regular purchase for the labs at a reasonable price.Images @10x magnification show limited differentiation of iPSCs after 3 days in culture.
Excellent product.
By
Jack Schnell on 03/15/2023
分析类型： In Vitro
种属： Human
细胞系/组织： iPSCs
We add this product at a concentration of 10 uM to promote cell survival in in vitro iPSC cultures.
Easy to manage and works as expected
ROCK Inhibitor.
By
Anonymous on 09/07/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Organoids
To generate human intestinal organoids
test.
By
Veselina Petrova on 08/28/2020
分析类型： In Vitro
种属： Human
I used this product on freshly split and plate human iPSC cells to help cell survival and adherence. I used the product at 10 mM concentration which I diluted in 1:1000 in culture media and left the cells at 37C, 5% CO2 for 24 hours before replacing with fresh culture media.
For hPSC culture.
By
Anonymous on 06/30/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Human PSC
For hPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS. Make 100µl aliquots and keep them at −20 °C for up to 6 months.
Worked great and at a great price.
By
Tres Davis on 05/08/2020
分析类型： Ex Vivo
种属： Mouse
HPLC
Neural Induction Conditions.
By
Anonymous on 03/22/2020
分析类型： In Vitro
种属： Human
细胞系/组织： H9
ROCK inhibitor Y-27632 (10 mM)
Great for passaging stem cells..
By
Samiha Shaikh on 01/31/2020
分析类型： In Vitro
种属： Human
细胞系/组织： iPSC
Great for passaging huiPSCs with dissociation into single cells.
Great for single iPS cell survival.
By
Anonymous on 09/13/2019
分析类型： In Vitro
种属： Human
细胞系/组织： iPSC
Used during single cell passage for CRISPR clone isolation, works well and no difference found between multiple batches. Cells show characteristic 'spiky' morphology upon addition to the medium.
I use at 1:1000 and store at -20C. I remove the compound from medium 24 hours after plating.
For iPSC culture.
By
Pinyuan Tian on 08/07/2018
分析类型： In Vitro
种属： Human
细胞系/组织： iPSC
For iPSC culture, final concentration 10mM. Reconstitute 10 mg of Y27632 in 3.0792 ml of PBS.
Make 100µl aliquots and keep them at −20 °C for up to 6 months.
ROCK Inhibitor to improve cartilage integrity.
By
Anonymous on 03/26/2018
分析类型： In Vitro
种属： Other
细胞系/组织： chondrocyte
I dissolved in DMSO
I prepare 20mg of the product in 5.88mL H2O and I use 1uL/mL of the stock in my cell culture after the cell splitting.
By
Anonymous on 02/01/2018
分析类型： In Vitro
种属： Human
细胞系/组织： human foreskin fibroblasts-derived-IPS
I used it in two human IPS cell lines, 5uM concentration the first 24 h after the splitting. The use of this reagent improves substantially the cell survival.
Keep the stock frozen at -20°C. I use it freshly added to the medium when splitting the cells.
ROCK Inhibitor to improve cell survival.
By
Hudson DE OLIVEIRA BEZERRA on 01/05/2018
分析类型： In Vitro
种属： Human
细胞系/组织： H1 (W01)
My team uses this product to improve human embryonic stem cells survival in single cell passage methods in siRNA and CRISPR experiments. In general, we use the concentration of 10 uM. The products work very well, better than others available in the market.
Y-27632 dihydrochloride 实验方案
The following protocols feature additional information for the use of Y-27632 dihydrochloride (Cat. No. 1254).
- Transdifferentiating Fibroblasts into Neurons
- Converting Fibroblasts into Cardiomyocytes (9C Cocktail)
- Accelerated Induction of Cortical Neurons from hiPSCs
- Human iPSC-derived Myoblasts
- Generation and Propagation of EPS cells (LCDM Cocktail)
- Cultivating Cerebral Organoids
- Generating Vascular Organoids
- 3D Culture of Lung Alveolar Cells
- Generating Midbrain Dopaminergic Neurons from hPSCs
该领域的文献
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*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
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Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
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