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Submit ReviewNutlin 3a
Description: MDM2 antagonist; active enantiomer of Nutlin-3 (Cat. No. 3984)
Chemical Name: 4-[[(4S,5R)-4,5-Bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl]-2-piperazinone
Purity: ≥97% (HPLC)
Biological Activity for Nutlin 3a
Nutlin 3a is an MDM2 antagonist (IC50 = 90 nM); inhibits the MDM2-p53 interaction. Active enantiomer of Nutlin-3 (Cat. No. 3984). Induces expression for P53 regulated genes. Suppresses tumor growth in vivo >98% in prostate cancer and osteosarcoma cancer models. Antiproliferative.
Compound Libraries for Nutlin 3a
Nutlin 3a is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.
Technical Data for Nutlin 3a
| M. Wt | 581.49 |
| Formula | C30H30Cl2N4O4 |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 675576-98-4 |
| PubChem ID | 11433190 |
| InChI Key | BDUHCSBCVGXTJM-WUFINQPMSA-N |
| Smiles | ClC1=CC=C([C@H]2[C@H](N(C(N3CC(NCC3)=O)=O)C(C4=CC=C(OC)C=C4OC(C)C)=N2)C5=CC=C(Cl)C=C5)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nutlin 3a
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 58.15 | 100 | |
| ethanol | 58.15 | 100 |
Preparing Stock Solutions for Nutlin 3a
The following data is based on the product molecular weight 581.49. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.72 mL | 8.6 mL | 17.2 mL |
| 5 mM | 0.34 mL | 1.72 mL | 3.44 mL |
| 10 mM | 0.17 mL | 0.86 mL | 1.72 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.34 mL |
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Product Datasheets for Nutlin 3a
References for Nutlin 3a
References are publications that support the biological activity of the product.
Tovar et al (2006) Small-molecule MDM2 antagonists reveal aberrant p53 signaling in cancer: implications for therapy. Proc.Natl.Acad.Sci.USA. 103 1888 PMID: 16443686
Vassilev et al (2004) In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science 303 844 PMID: 14704432
If you know of a relevant reference for Nutlin 3a, please let us know.
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Keywords: Nutlin 3a, Nutlin 3a supplier, Nutlin3a, p53, activators, MDM2, antagonism, antagonists, p53-MDM2, activates, Ubiquitin, E3, Ligases, 6075, Tocris Bioscience
2 Citations for Nutlin 3a
Citations are publications that use Tocris products. Selected citations for Nutlin 3a include:
Slobodan et al (2020) Embryonic Barcoding of Equipotent Mammary Progenitors Functionally Identifies Breast Cancer Drivers. Cell Stem Cell 26 403-419.e4 PMID: 32059806
Catherine et al (2021) p53 mitigates the effects of oncogenic HRAS in urothelial cells via the repression of MCOLN1. iScience 24 102701 PMID: 34222845
Do you know of a great paper that uses Nutlin 3a from Tocris? Please let us know.
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Literature in this Area
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TPD and Induced Proximity Research Product GuideUpdated
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