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Submit ReviewCHIR 99021
GMP Version Available:
CHIR 99021
说明： Highly selective GSK-3 inhibitor; acts as Wnt activator
别名： CHIR99021,CT99021
化学名： 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
纯度： ≥98% (HPLC)
生物活性 for CHIR 99021
CHIR 99021 is a potent and highly selective inhibitor of glycogen synthase kinase 3 (GSK-3) (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively). Acts as Wnt activator. Exhibits >500-fold selectivity for GSK-3 over closely related kinases; also displays >800-fold selectivity against 45 additional enzymes and receptors. In combination with the irreversible inhibitor of LSD1 (Cat. No. 3852), CHIR 99021 enables reprogramming of mouse embryonic fibroblasts, transduced by Oct4 and Klf4 only, into iPSCs. Enhances mouse and human ESC self-renewal when used in combination with PD 0325901 (Cat. No. 4192). It promotes differentiation of hPSCs in cardiomyocytes and allows formation of extended pluripotent stem (EPS) cells in combination with (S)-(+)-Dimethindene maleate (Cat. No. 1425), tetracycline antibiotic (Cat. No. 3268) and human leukemia inhibitory factor. Used in a protocol to generate brain organoids from human iPSCs. Also used in iPS cell differentiation protocol that produces CD34+ hematopoietic cells with multilineage engraftment ability.
CHIR 99021 synthesized to cGMP guidelines and CHIR 99021 in solution also available.
For more information on how to use CHIR 99021, see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts.
Scientific Data
Cardiomyocyte derived from human induced pluripotent stem cells (hiPSCs) using CHIR 99021. Cells cultured in Media supplemented with Activin A (Catalog # 338-AC, R&D Systems), N21-MAX Insulin Free (Catalog # AR010, R&D Systems), BMP-4 (Catalog # 314-BP, R&D Systems), bFGF (Catalog # 233-FB, R&D Systems) and DKK-1 (Catalog # 5439-DK, R&D Systems). Stained for F-Actin (Phalloidin, green), Troponin T (red, Human Troponin T (Cardiac) Antibody, Catalog # MAB1874, R&D Systems) and counterstained with DAPI (blue, Catalog # 5748, Tocris).
External Portal Information for CHIR 99021
Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of CHIR-99021 is reviewed on the chemical probes website.
化合物库 for CHIR 99021
CHIR 99021 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. 了解 Tocris 化合物库的更多信息。
技术数据 for CHIR 99021
| 分子量 | 465.34 |
| 公式 | C22H18Cl2N8 |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 252917-06-9 |
| PubChem ID | 9956119 |
| InChI Key | AQGNHMOJWBZFQQ-UHFFFAOYSA-N |
| Smiles | ClC(C=C1Cl)=CC=C1C2=NC(NCCNC3=CC=C(C#N)C=N3)=NC=C2C4=NC=C(C)N4 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for CHIR 99021
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 9.31 | 20 |
制备储备液 for CHIR 99021
以下数据基于产品分子量 465.34。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 10.74 mL | 53.72 mL | 107.45 mL |
| 1 mM | 2.15 mL | 10.74 mL | 21.49 mL |
| 2 mM | 1.07 mL | 5.37 mL | 10.74 mL |
| 10 mM | 0.21 mL | 1.07 mL | 2.15 mL |
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产品说明书 for CHIR 99021
参考文献 for CHIR 99021
参考文献是支持产品生物活性的出版物。
Pan et al (2011) AKT kinase activity is required for Li to modulate mood-related behaviors in mice. Neuropsychopharmacology 36 1397 PMID: 21389981
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Ye et al (2012) Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embyronic stem cells from refractory mouse strains. PLoS One 7 e35892 PMID: 22540008
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Ng et al (2024) Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells. Nat.Biotechnol. PMID: 39223325
If you know of a relevant reference for CHIR 99021, please let us know.
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关键词: CHIR 99021, CHIR 99021 supplier, CHIR99021, glycogen, synthase, kinase, 3, gsk-3, gsk-3a, gsk-3alpha, gsk-3α, gsk-3b, gsk-3beta, gsk-3β, inhibitors, inhibits, highly, selective, potent, organoids, Wnt, agonist, activator, CHIR, CT99021, CT, 99021, hematopoietic, haematopoietic, stem, cells, HSC, CD34+, multi, Glycogen, Synthase, Kinase, Stem, Cell, Reprogramming, Organoids, Hematopoietic, Cells, 4423, Tocris Bioscience
412 篇 CHIR 99021 的引用文献
引用文献是使用了 Tocris 产品的出版物。 CHIR 99021 的部分引用包括：
Carissa et al (2020) Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development. Dev Cell 53 229-239.e7 PMID: 32197069
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Janghwan et al (2020) The combination of forskolin and VPA increases gene expression efficiency to the hypoxia/neuron-specific system. Ann Transl Med 8 933 PMID: 32953733
Lee J et al (2020) Human Motor Neurons With SOD1-G93A Mutation Generated From CRISPR/Cas9 Gene-Edited iPSCs Develop Pathological Features of Amyotrophic Lateral Sclerosis. Front Cell Neurosci 14 604171 PMID: 33328898
Micha et al (2020) Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation. BMC Biol 18 42 PMID: 32321486
Chan Young et al (2020) Robust and Reproducible Generation of Induced Neural Stem Cells from Human Somatic Cells by Defined Factors. Int J Stem Cells 13 80-92 PMID: 32114739
Joshua M et al (2020) A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract. Sci Adv 6 PMID: 33268364
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Jianming et al (2020) Human Bocavirus 1 Infection of Well-Differentiated Human Airway Epithelium. Curr Protoc Microbiol 58 e107 PMID: 32639683
Steven C et al (2020) Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency. Cell Stem Cell 27 459-469.e8 PMID: 32795400
Vijay Pratap et al (2020) Persistent DNA Damage and Senescence in the Placenta Impacts Developmental Outcomes of Embryos. Dev Cell 54 333-347.e7 PMID: 32800293
Andrew et al (2020) Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells. Cell Stem Cell 27 663-678.e8 PMID: 32891189
Igor I et al (2020) Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells. Bio Protoc 10 e3675 PMID: 33659345
Mou et al (2016) Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells Cell: Stem Cell 19 217 PMID: 27320041
Lu et al (2016) Generation of serotonin neurons from human pluripotent stem cells. Nat Biotechnol 34 89 PMID: 26655496
Capowski et al (2016) Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells. Stem Cells 34 2625 PMID: 27301076
Zimmerlin et al (2016) Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality. Development 143 4368 PMID: 27660325
Lin et al (2016) HP Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med PMID: 27591141
Shelton et al (2014) Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells. Dis Model Mech 3 516 PMID: 25241748
Wu et al (2014) A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation. Stem Cell Res 289 20193 PMID: 24895131
Skelton et al (2014) SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development. J Cachexia Sarcopenia Muscle 13 172 PMID: 24968096
Murase et al (2024) In vitro reconstitution of epigenetic reprogramming in the human germ line. Nature PMID: 38768632
Yang et al (2024) Metformin decelerates aging clock in male monkeys. Cell 187 1 PMID: 39270656
Ng et al (2024) Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells. Nat.Biotechnol. PMID: 39223325
Laween et al (2023) Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds. Nat Protoc 18 108-135 PMID: 36261633
Bo et al (2023) Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing. iScience 26 105898 PMID: 36647382
Stefan et al (2023) Pump-less, recirculating organ-on-a-chip (rOoC) platform. Lab Chip 23 591-608 PMID: 36655405
Ludo et al (2023) FUS-ALS hiPSC-derived astrocytes impair human motor units through both gain-of-toxicity and loss-of-support mechanisms. Mol Neurodegener 18 5 PMID: 36653804
Serena et al (2023) Automated high-content imaging in iPSC-derived neuronal progenitors. SLAS Discov 28 42-51 PMID: 36610640
C Frank et al (2023) Long-Term SMN- and Ncald-ASO Combinatorial Therapy in SMA Mice and NCALD-ASO Treatment in hiPSC-Derived Motor Neurons Show Protective Effects. Int J Mol Sci 24 PMID: 36835624
Sara et al (2023) Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation. Nat Cell Biol 25 481-492 PMID: 36690849
Mustafa et al (2023) A Robust Pipeline for the Multi-Stage Accelerated Differentiation of Functional 3D Cortical Organoids from Human Pluripotent Stem Cells. Curr Protoc 3 e641 PMID: 36633423
Qian et al (2023) Spatial multiomics map of trophoblast development in early pregnancy. Nature 616 143-151 PMID: 36991123
Yulia V et al (2023) Engineered cardiac tissue model of restrictive cardiomyopathy for drug discovery. Cell Rep Med 4 100976 PMID: 36921598
Steven P et al (2023) Genetic dissection of the pluripotent proteome through multi-omics data integration. Cell Genom 3 100283 PMID: 37082146
Jie et al (2023) Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids. Bio Protoc 13 e4657 PMID: 37113328
Dmitry et al (2023) Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance. Int J Mol Sci 24 PMID: 37047264
Mark et al (2023) Axon guidance genes modulate neurotoxicity of ALS-associated UBQLN2. Elife 12 PMID: 37039476
Stefan et al (2023) Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging. Cell Rep Methods 3 100440 PMID: 37159662
Nadine et al (2023) A distal lung organoid model to study interstitial lung disease, viral infection and human lung development. Nat Protoc PMID: 37165073
Alessandro et al (2023) Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells. Cells 12 PMID: 37190056
Tim et al (2023) A pesticide and iPSC dopaminergic neuron screen identifies and classifies Parkinson-relevant pesticides. Nat Commun 14 2803 PMID: 37193692
Jing et al (2023) Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. NPJ Regen Med 8 26 PMID: 37236990
Aditya et al (2023) Systematic discovery of transcription factors that improve hPSC-derived cardiomyocyte maturation via temporal analysis of bioengineered cardiac tissues. APL Bioeng 7 026109 PMID: 37252678
Alessandra et al (2023) Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models. Front Cell Dev Biol 11 1111684 PMID: 37261075
Bouffi et al (2023) In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice Nat.Biotechnol. PMID: 36702898
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Amos et al (2020) SEM/FIB Imaging for Studying Neural Interfaces. Dev Neurobiol 80 305-315 PMID: 31228876
Narutoshi et al (2020) Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting. Methods Mol Biol 2140 183-197 PMID: 32207113
Krisztina et al (2020) Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos. Methods Mol Biol 2258 131-147 PMID: 33340359
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Mark et al (2020) Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity. Cell Stem Cell 27 813-821.e6 PMID: 32931730
Masayuki et al (2020) Intracellular pH controls WNT downstream of glycolysis in amniote embryos. Nature 584 98-101 PMID: 32581357
Alexander R et al (2020) Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms. J Vac Sci Technol A 38 033205 PMID: 32255900
Michael A et al (2020) Differentiation of PTH-Expressing Cells From Human Pluripotent Stem Cells. Endocrinology 161 PMID: 32810225
Joshy et al (2020) Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. Mol Cell 80 648-665.e9 PMID: 33176162
Emanuela et al (2020) Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development PMID: 34004948
Maxime M et al (2020) Pluripotent stem cell derived intestinal organoids with an enteric nervous system. Methods Cell Biol 159 175-199 PMID: 32586442
Kotaro et al (2020) Generation of human oogonia from induced pluripotent stem cells in culture. Nat Protoc 15 1560-1583 PMID: 32231324
Nicholas C et al (2020) Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells. Curr Protoc Immunol 131 e113 PMID: 33166041
Lucy et al (2020) Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta. Nat Protoc 15 3441-3463 PMID: 32908314
Donna L et al (2020) SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27 951-961.e5 PMID: 33113348
Hyun Jung et al (2020) Neural Transplants From Human Induced Pluripotent Stem Cells Rescue the Pathology and Behavioral Defects in a Rodent Model of Huntington's Disease. Front Neurosci 14 558204 PMID: 33071737
Thaddeus G et al (2020) Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep 10 19159 PMID: 33154556
Lisa M et al (2020) FOXO3 targets are reprogrammed as Huntington's disease neural cells and striatal neurons face senescence with p16INK4a increase. Aging Cell 19 e13226 PMID: 33156570
Alon et al (2020) Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors. Invest Ophthalmol Vis Sci 61 44 PMID: 32991686
Richard A et al (2020) Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell 27 822-839.e8 PMID: 32946788
Malcolm G et al (2020) Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1. Cell Mol Gastroenterol Hepatol 11 465-489 PMID: 32971322
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Ludovic et al (2020) Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation. Cell Stem Cell 27 470-481.e6 PMID: 32795399
Adrian S et al (2020) Aberrant Differentiation of Human Pluripotent Stem Cell-Derived Kidney Precursor Cells inside Mouse Vascularized Bioreactors. Nephron 144 509-524 PMID: 32756058
Frédéric H-T et al (2020) Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis. Nat Commun 11 6341 PMID: 33311468
April D et al (2020) Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells. STAR Protoc 1 100158 PMID: 33377052
Emanuela et al (2020) Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development 147 PMID: 32541004
Constanze et al (2020) Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells. STAR Protoc 1 100130 PMID: 33377024
Koen C et al (2020) Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids. STAR Protoc 1 100126 PMID: 33377020
Edwin M et al (2020) Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy. Am J Hum Genet 107 278-292 PMID: 32707085
Takashi et al (2020) Hedgehog Activation Regulates Human Osteoblastogenesis. Stem Cell Reports 15 125-139 PMID: 32531191
Peter W et al (2020) Nucleosides Rescue Replication-Mediated Genome Instability of Human Pluripotent Stem Cells. Stem Cell Reports 14 1009-1017 PMID: 32413278
Jizhong et al (2020) Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions. STAR Protoc 1 PMID: 32734277
Mary Lou et al (2020) SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response. Cell Stem Cell 27 962-973.e7 PMID: 32979316
Peter et al (2020) Evaluating the Effects of Cell Sorting on Gene Expression. J Biomol Tech 31 100-111 PMID: 32982601
Rui et al (2020) Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause. Nat Commun 11 5499 PMID: 33127892
Ting et al (2020) Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9. STAR Protoc 1 PMID: 33073252
Gareth et al (2020) A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates. Sci Rep 10 18498 PMID: 33116175
Hui et al (2020) 16p11.2 microdeletion imparts transcriptional alterations in human iPSC-derived models of early neural development. Elife 9 PMID: 33169669
Byeong-Sun et al (2020) Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell 27 905-919.e10 PMID: 33142113
Richard C et al (2020) Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction. Cell Stem Cell 27 890-904.e8 PMID: 33128895
Darrell N et al (2020) Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells. Nat Commun 11 215 PMID: 31924806
Qianqian et al (2020) A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG. Stem Cell Res Ther 11 67 PMID: 32070424
Preethi et al (2020) High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis. Cell Rep 30 2055-2064.e5 PMID: 32075752
Sun et al (2020) Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives. Sci Rep 10 3939 PMID: 32127560
Jeong-Eun et al (2020) Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke. Stem Cells Int 2020 4061516 PMID: 32269595
Robert et al (2020) CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual. Commun Biol 3 33 PMID: 31959876
Xavier et al (2020) BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11 378 PMID: 32424151
Constanze et al (2020) Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells. Cell Rep 31 107691 PMID: 32460028
Peter et al (2020) Evaluating the Effects of Cell Sorting on Gene Expression. J Biomol Tech PMID: 32831654
Paul et al (2020) A Non-Coding Disease Modifier of Pancreatic Agenesis Identified by Genetic Correction in a Patient-Derived iPSC Line. Cell Stem Cell 27 137-146.e6 PMID: 32442395
Katja et al (2020) A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Cell Stem Cell 27 158-176.e10 PMID: 32396864
Da-Zhi et al (2020) Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells. EMBO J 39 e104324 PMID: 32614092
Valentina et al (2020) Intravital three-dimensional bioprinting. Nat Biomed Eng 4 901-915 PMID: 32572195
Yanyan et al (2020) Autologous correction in patient induced pluripotent stem cell-endothelial cells to identify a novel pathogenic mutation of hereditary hemorrhagic telangiectasia. Pulm Circ 10 2045894019885357 PMID: 33282178
Oliver et al (2020) Inhibition of LTβR signalling activates WNT-induced regeneration in lung. Nature 588 151-156 PMID: 33149305
Tina et al (2020) An in vitro model of early anteroposterior organization during human development. Nature 582 410-415 PMID: 32528178
Christoph et al (2020) In vitro characterization of the human segmentation clock. Nature 580 113-118 PMID: 31915384
Yuka et al (2020) Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nat Commun 11 5656 PMID: 33168808
Claudio J et al (2020) Regulation of Tumor Initiation by the Mitochondrial Pyruvate Carrier. Cell Metab 31 284-300.e7 PMID: 31813825
Melissa J et al (2020) Genome Editing-Mediated Utrophin Upregulation in Duchenne Muscular Dystrophy Stem Cells. Mol Ther Nucleic Acids 22 500-509 PMID: 33230452
Jason et al (2020) Generation and trapping of a mesoderm biased state of human pluripotency. Nat Commun 11 4989 PMID: 33020476
Sheng et al (2020) Overcoming Wnt-β-catenin dependent anticancer therapy resistance in leukaemia stem cells. Nat Cell Biol 22 689-700 PMID: 32313104
Olivier et al (2020) Bioelectrical domain walls in homogeneous tissues. Nat Phys 16 357-364 PMID: 33790984
Lorna et al (2020) Differentiation of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell-Derived Ureteric Bud and Ex Fetu Mesenchyme. J Am Soc Nephrol 31 2253-2262 PMID: 32826325
Vermilyea et al (2017) Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts. Stem Cells Dev 26 1225 PMID: 28635509
Sampaziotis et al (2017) Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Nat Protoc 12 814 PMID: 28333915
McCauley et al (2017) Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling. Cell Stem Cell 20 844 PMID: 28366587
Lancaster et al (2017) Guided self-organization and cortical plate formation in human brain organoids. Nat Biotechnol 35 659 PMID: 28562594
Morizane and Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc 12 195 PMID: 28005067
Yang et al (2017) Establishment of mouse expanded potential stem cells. Nature 550 393 PMID: 29019987
Ong et al (2017) Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. Sci Rep 7 4566 PMID: 28676704
Kumar et al (2017) Dynamic regulation of Nanog and stem cell-signaling pathways by Hoxa1 during early neuro-ectodermal differentiation of ES cells. Proc Natl Acad Sci U S A 114 5838 PMID: 28584089
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Kenneth et al (2014) Transcriptional profiling of Wnt3a mutants identifies Sp transcription factors as essential effectors of the Wnt/β-catenin pathway in neuromesodermal stem cells. PLoS One 9 e87018 PMID: 24475213
Yoon-Young et al (2014) In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. Methods Mol Biol 1353 271-83 PMID: 25520290
Peter et al (2014) Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells. Nat Protoc 9 1514-31 PMID: 24874816
Lorenz et al (2013) Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells. Methods Mol Biol 1307 329-43 PMID: 24301074
Wendy et al (2022) The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells. Elife 11 PMID: 35815953
Sandeep et al (2022) In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion. Cell Rep 40 111119 PMID: 35858555
Diego et al (2022) Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord. Eneuro 9 PMID: 36265906
Kamil et al (2022) Engineering a living cardiac pump on a chip using high-precision fabrication. Sci Adv 8 eabm3791 PMID: 35452278
Marc et al (2022) Three-Dimensional Vessels-on-a-Chip Based on hiPSC-derived Vascular Endothelial and Smooth Muscle Cells. Curr Protoc 2 e564 PMID: 36250774
Michael et al (2022) Transplanted organoids empower human preclinical assessment of drug candidate for the clinic. Sci Adv 8 eabj5633 PMID: 35857479
Elisabeth et al (2022) Autophagy modulates cell fate decisions during lineage commitment. Autophagy 18 1915-1931 PMID: 34923909
Young-Woock et al (2022) Human Pluripotent Stem Cell-Derived Alveolar Organoid with Macrophages. Int J Mol Sci 23 PMID: 36012471
Sallie et al (2022) Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface. Elife 11 PMID: 35975985
Xi et al (2022) EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation. Elife 11 PMID: 35983994
Jorge et al (2022) Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Dev Cell 57 1922-1936.e9 PMID: 35998583
Xi et al (2022) An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions. STAR Protoc 3 101918 PMID: 36595937
Jay K et al (2022) IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment. Immunity 55 237-253.e8 PMID: 35081371
Ludovic et al (2022) Organoids capture tissue-specific innate lymphoid cell development in mice and humans. Cell Rep 40 111281 PMID: 36044863
Christine E et al (2022) Transcription factor protein interactomes reveal genetic determinants in heart disease. Cell 185 794-814.e30 PMID: 35182466
Cheng et al (2022) Genome-wide identification of the genetic basis of amyotrophic lateral sclerosis. Neuron 110 992-1008.e11 PMID: 35045337
Timothy C et al (2022) Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells. Elife 11 PMID: 36129169
Florent et al (2022) Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses. Viruses 15 PMID: 36680128
Francisco J et al (2022) Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells. Nat Commun 13 7470 PMID: 36463236
Emmanouil et al (2022) Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled NEUROG2 overexpression. Cell Rep Methods 2 100341 PMID: 36452863
Elly et al (2022) Canonical Wnt signaling and the regulation of divergent mesenchymal Fgf8 expression in axolotl limb development and regeneration. Elife 11 PMID: 35587651
Yin et al (2022) Novel GSK-3 kinase inhibitor Pym-5 induces GSK-3β rather than GSK-3α-dependent melanogenesis in murine melanoma cells. J Dermatol Sci 106 170-180 PMID: 35641396
Janet L et al (2022) Generation of human elongating multi-lineage organized cardiac gastruloids. STAR Protoc 3 101898 PMID: 36595961
Kalpaj R et al (2022) A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas. Cells 11 PMID: 35326478
Mustafa et al (2022) Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities. Curr Protoc 2 e568 PMID: 36264199
Catherine et al (2022) DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagy. Brain 145 4368-4384 PMID: 36039535
Ran et al (2022) Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signaling. Elife 11 PMID: 35394424
James R et al (2022) Human induced pluripotent stem cells integrate, create synapses and extend long axons after spinal cord injury. J Cell Mol Med 26 1932-1942 PMID: 35257489
Angelika et al (2022) Differentiation of iPS-Cells into Peripheral Sensory Neurons. Methods Mol Biol 2429 175-188 PMID: 35507161
Reza et al (2022) In Vitro Generation of Heart Field Specific Cardiomyocytes. Methods Mol Biol 2429 257-267 PMID: 35507167
Chen et al (2022) Ti3 C2 Tx MXene Composite 3D Hydrogel Potentiates mTOR Signaling to Promote the Generation of Functional Hair Cells in Cochlea Organoids. Adv Sci (Weinh) 9 e2203557 PMID: 36117048
Bruce et al (2022) Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells. Nat Cell Biol 24 10-23 PMID: 34969962
Roy et al (2022) Kidney organoids recapitulate human basement membrane assembly in health and disease. Elife 11 PMID: 35076391
Bong-Kiun et al (2022) The GSK-3 Inhibitor CT99021 Enhances the Acquisition of Spatial Learning and the Accuracy of Spatial Memory. Front Mol Neurosci 14 804130 PMID: 35153671
Gordon F et al (2022) Development of high-affinity nanobodies specific for NaV1.4 and NaV1.5 voltage-gated sodium channel isoforms. J Biol Chem 298 101763 PMID: 35202650
Martin et al (2022) Metabolomic profiling of human pluripotent stem cell differentiation into lung progenitors. iScience 25 103797 PMID: 35198866
Takashi et al (2022) Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports 17 820-834 PMID: 35245440
Shyni et al (2022) An In Vitro Microfluidic Alveolus Model to Study Lung Biomechanics. Front Bioeng Biotechnol 10 848699 PMID: 35252157
Miguel N et al (2022) A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine. Nat Commun 13 1503 PMID: 35314684
Christine et al (2022) Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation. Sci Rep 12 4953 PMID: 35322081
Catherine et al (2022) Engineering neurovascular organoids with 3D printed microfluidic chips. Lab Chip 22 1615-1629 PMID: 35333271
Chengkai et al (2022) A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication. Bioact Mater 18 138-150 PMID: 35387155
Hong et al (2022) Spinal dI4 Interneuron Differentiation From Human Pluripotent Stem Cells. Front Mol Neurosci 15 845875 PMID: 35465095
Stefan et al (2022) The antimicrobial peptide S100A8/A9 produced by airway epithelium functions as a potent and direct regulator of macrophage phenotype and function. Eur Respir J 59 PMID: 34561292
Guojie et al (2022) JAK inhibitor blocks COVID-19 cytokine-induced JAK/STAT/APOL1 signaling in glomerular cells and podocytopathy in human kidney organoids. JCI Insight 7 PMID: 35472001
Jing et al (2022) Single-cell atlas of the aging mouse colon. iScience 25 104202 PMID: 35479413
Monica M et al (2022) Re-Expression of ERα and AR in Receptor Negative Endocrine Cancers via GSK3 Inhibition. Front Oncol 12 824594 PMID: 35402240
Alexandre P et al (2022) An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates. Elife 11 PMID: 35536602
Yue et al (2022) Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response. Redox Biol 50 102248 PMID: 35091324
Xingbin et al (2022) Primary culture of tracheal aspirate-derived human airway basal stem cells. STAR Protoc 3 101390 PMID: 35600918
Yoshihiro et al (2022) CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells. iScience 25 104314 PMID: 35602937
Fan et al (2022) Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotection. iScience 25 104447 PMID: 35707727
Hiromitsu et al (2022) Streamlined and quantitative detection of chimerism using digital PCR. Sci Rep 12 10223 PMID: 35715477
Aleksandra et al (2022) Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc 3 101447 PMID: 35712012
Janet L et al (2022) A combined human gastruloid model of cardiogenesis and neurogenesis. iScience 25 104486 PMID: 35721464
Yen-Wen et al (2022) Tumor endothelial marker 1 is upregulated in heart after cardiac injury and participates in cardiac remodeling. Sci Rep 12 10532 PMID: 35732643
Wenqi et al (2022) Reengineering Ponatinib to Minimize Cardiovascular Toxicity. Cancer Res 82 2777-2791 PMID: 35763671
Vincent et al (2022) Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development. Nat Commun 13 3861 PMID: 35790717
Mary Lou et al (2022) A multimodal iPSC platform for cystic fibrosis drug testing. Nat Commun 13 4270 PMID: 35906215
Muhammad Abid et al (2022) Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features. Cell Death Dis 13 670 PMID: 35915082
Erica et al (2022) Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers. Elife 11 PMID: 35920628
Stephanie et al (2022) Protocols to culture and harvest hepatic tumor organoids for metabolic assays. STAR Protoc 3 101597 PMID: 35942348
Beate et al (2022) Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease. Nat Commun 13 4819 PMID: 35974013
Gordana et al (2022) STK25 inhibits PKA signaling by phosphorylating PRKAR1A. Cell Rep 40 111203 PMID: 35977512
Zhen et al (2022) A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production. Nat Commun 13 5491 PMID: 36123354
Peng et al (2022) Isolation and characterization of human embryonic stem cell-derived heart field-specific cardiomyocytes unravels new insights into their transcriptional and electrophysiological profiles. Cardiovasc Res 118 828-843 PMID: 33744937
Lu et al (2022) Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nat Protoc 17 2699-2719 PMID: 35978039
Kyung-Sun et al (2022) Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes. iScience 25 105150 PMID: 36193049
Sagrario et al (2022) The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A. Nat Cell Biol 24 1528-1540 PMID: 36202974
Simone et al (2022) Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro. iScience 25 105197 PMID: 36238899
Chong-Shan et al (2022) LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells. Autophagy 18 204-222 PMID: 34313548
Eva et al (2019) Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes. Bio Protoc 9 e3312 PMID: 33654821
Konstantinos-Dionysios et al (2019) Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells. Nat Protoc 14 3303-3332 PMID: 31732721
Tracy C et al (2019) Wnt signaling inhibition by monensin results in a period of Hippo pathway activation during intestinal adaptation in zebrafish. Am J Physiol Gastrointest Liver Physiol 316 G679-G691 PMID: 30896968
Ryan M et al (2019) Specification of positional identity in forebrain organoids. Nat Biotechnol 37 436-444 PMID: 30936566
Kevin et al (2019) Derivation of enteric neuron lineages from human pluripotent stem cells. Nat Protoc 14 1261-1279 PMID: 30911172
Kyoung Hwa et al (2019) Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm. J Cell Sci 132 PMID: 31492758
Olga et al (2019) Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J Vis Exp PMID: 31033964
Puspa R et al (2019) End-to-End Platform for Human Pluripotent Stem Cell Manufacturing. Int J Mol Sci 21 PMID: 31877727
Fan et al (2019) Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells. Int J Mol Sci 21 PMID: 31877913
Francois et al (2019) Cortical Neurogenesis Requires Bcl6-Mediated Transcriptional Repression of Multiple Self-Renewal-Promoting Extrinsic Pathways. Neuron 103 1096-1108.e4 PMID: 31353074
Chi-chung et al (2019) Activation of Hedgehog Signaling Promotes Development of Mouse and Human Enteric Neural Crest Cells, Based on Single-Cell Transcriptome Analyses. Gastroenterology 157 1556-1571.e5 PMID: 31442438
David J et al (2019) The role of cardiac transcription factor NKX2-5 in regulating the human cardiac miRNAome. Sci Rep 9 15928 PMID: 31685864
Ajeet P et al (2019) Enteroendocrine Progenitor Cell-Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner. Cell Mol Gastroenterol Hepatol 9 447-464 PMID: 31756561
James R et al (2019) Cardiomyocyte functional screening: interrogating comparative electrophysiology of high-throughput model cell systems. Am J Physiol Cell Physiol 317 C1256-C1267 PMID: 31577512
Guangming et al (2019) Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cell 25 737-753.e4 PMID: 31708402
Steven M et al (2019) Cardiac regeneration using human-induced pluripotent stem cell-derived biomaterial-free 3D-bioprinted cardiac patch in vivo. J Tissue Eng Regen Med 13 2031-2039 PMID: 31408915
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Sebastian et al (2019) Development of MAP4 Kinase Inhibitors as Motor Neuron-Protecting Agents. Cell Chem Biol 26 1703-1715.e37 PMID: 31676236
Peidong et al (2019) Canonical Wnt5b Signaling Directs Outlying Nkx2.5+ Mesoderm into Pacemaker Cardiomyocytes. Dev Cell 50 729-743.e5 PMID: 31402282
Matthew W et al (2019) GSK3 regulates hair cell fate in the developing mammalian cochlea. Dev Biol 453 191-205 PMID: 31185200
Marc et al (2019) Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis. Nat Med 25 1873-1884 PMID: 31806906
Martín I et al (2019) Human neural crest induction by temporal modulation of WNT activation. Dev Biol 449 99-106 PMID: 30826399
Thomas C et al (2019) Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity. Cell Stem Cell 25 103-119.e6 PMID: 31155484
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IPS culture.
By
Murali K Yanda on 09/27/2022
分析类型： In Vitro
种属： Mouse
细胞系/组织： ips
Mouse ips cells culture with 2i media.
2i medium culture.
By
Yu Chen on 07/11/2022
分析类型： In Vitro
种属： Mouse
细胞系/组织： ESC
ESC 2i medium culture; 3 uM
ESC culture.
By
Xiaoyu Zhang on 11/23/2021
分析类型： In Vitro
种属： Mouse
细胞系/组织： ESC
In vitro cell culture addition. The image shows the morphology of ESC colonies after conversion using the treatment condition. This condition supported the conversion and long-term maintenance of dome-shaped ESCs from primed ESCs.
Differentiation to cartilage.
By
Julieta O'Flaherty on 12/08/2020
分析类型： In Vitro
种属： Human
细胞系/组织： iPSCs
I used at 3uM to generate cartilage from iPSCs, worked really well. Keep at -80C for long term storage
Kidney organoids.
By
Kirsty Rooney on 11/21/2020
分析类型： In Vitro
种属： Human
细胞系/组织： MAN13 hESCs
I use 8uM of CHIR to differentiate hESCs to intermediate mesoderm and then to kidney organoids
Differentiation of human pluripotent stem cells.
By
Paul Humphreys on 11/20/2020
分析类型： In Vitro
种属： Human
细胞系/组织： hPSCs
Directed differentiation of human pluripotent stem cells (hPSCs) towards mesoderm lineages. Used at concentration of 3uM. Very effective in induction of mesoderm markers.
Perform titration against your cell line to optimise correct concentration.
iPSC differentiation.
By
Stephanie Hsiao on 10/13/2020
分析类型： In Vitro
种属： Human
细胞系/组织： iPSC
Differentiation of human iPSCs into hematopoietic progenitor cells.
iPSC culture medium supplemented with 4 uM of CHIR99021.
It worked good.
By
Anonymous on 07/04/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： iPSC
Reprogramming media comprises of 3 μM CHIR99021 and 0.5 μM A-83-01.
The cells were then maintained in RM 15 days with fresh media every other day
For maintenance of stem cells pluripotency.
By
Anonymous on 06/25/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： Neural Stem Cells
For directed differentiation to neural, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. This product gave me reproducible results.
It's better to aliquot and keep it at -80°C for long term storage.
Works as intended..
By
Anonymous on 04/04/2020
分析类型： In Vitro
种属： Rat
细胞系/组织： Sprague-Dawley rats primary neuronal
10 μM for 2 h
Differentiation of human dopaminergic neurons from stem cells.
By
Anonymous on 02/17/2020
分析类型： In Vitro
种属： Human
细胞系/组织： Stem Cells
Used this product routinely to differentiate dopaminergic neurons from human stem cells.
The hiPSCs were maintained with Essential 8 Flex Medium with CHIR 99021.
By
Anonymous on 02/08/2020
分析类型： In Vitro
种属： Human
细胞系/组织： ipsc
1.4 μM
All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901..
By
Anonymous on 12/04/2019
分析类型： In Vitro
种属： Mouse
细胞系/组织： ipsc
All iPSC lines and R1 mouse ES cells were maintained on feeders in KO-DMEM (Invitrogen) with 3 mM CHIR99021, and 1 mM PD0325901.
works great for Wnt activation.
By
avinash waghray on 08/08/2019
分析类型： In Vitro
种属： Human
In Tissue culture for growing basal cells
Check the maintenance of naive pluripotency in 2i.
By
Megha PB on 07/18/2018
分析类型： In Vitro
种属： Mouse
细胞系/组织： E14TG2a
Culture mouse ES cells in 2i media and without 2i to assess differentiation. Only the cells in 2i are pluripotent while mES cells cultured in -2i for 48 hours exit naive pluripotency.
reliable product.
By
Pinyuan Tian on 07/05/2018
分析类型： In Vitro
种属： Human
细胞系/组织： pluripotent stem cells
For directed differentiation of pluripotent stem cells to kidney organoids, cells are treated with GSK-3 inhibitor, CHIR99021 to induce canonical WNT signaling. CHIR99021 concentration and differentiation time period are the key factors for this protocol. I found this product gave me reproducible results.
Although the datasheet says store at -20°C, it's better to aliquot and keep it at -80°C for long term storage.
CHIR 99021 实验方案
The following protocols feature additional information for the use of CHIR 99021 (Cat. No. 4423).
- Transdifferentiating Fibroblasts into Neurons
- Culturing Transcriptomically Distinct Pluripotent mESCs
- Converting Fibroblasts into Cardiomyocytes (9C Cocktail)
- Human iPSC-derived Myoblasts
- Reprogramming MEFs into Skeletal Muscle Progenitors
- Highly Efficient Generation of CiPSCs from MEFs
- Generation of β cells from hPSCs
- Generation and Propagation of EPS cells (LCDM Cocktail)
- Generating Vascular Organoids
- 3D Culture of Lung Alveolar Cells
- 3D Culture of Lung Alveolar Cells
- Differentiation of Hematopoietic Progenitors from hPSCs
- Generating Midbrain Dopaminergic Neurons from hPSCs
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Alzheimer's Disease Poster
Alzheimer's disease (AD) is a debilitating and progressive neurodegenerative disease and the most common cause of dementia, affecting approximately 30% of individuals aged over 85 years. This poster summarizes the cellular and molecular mechanisms of AD.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.
Pathways for CHIR 99021
